Human COL1A1 (Collagen alpha-1 (I) chain) CLIA Kit (STJC0000435) STJC0000435 上海玉博生物科技有限公司

Human COL1A1 (Collagen alpha-1 (I) chain) CLIA Kit (STJC0000435)

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产品名称：Human COL1A1 (Collagen alpha-1 (I) chain) CLIA Kit (STJC0000435)
产品型号：STJC0000435
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human COL1A1 (Collagen alpha-1 (I) chain) CLIA Kit (STJC0000435)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This human COL1A1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	COL1A1
Gene ID:	1277
Uniprot ID:	CO1A1_HUMAN
Specificity:	This kit recognizes Human COL1 Alpha 1 in samples. No significant cross-reactivity or interference between Human COL1 Alpha 1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post Translational Modifications	Contains mostly 4-hydroxyproline. Proline residues at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. Contains 3-hydroxyproline at a few sites. This modification occurs on the first proline residue in the sequence motif Gly-Pro-Hyp, where Hyp is 4-hydroxyproline. Lysine residues at the third position of the tripeptide repeating unit (G-X-Y) are 5-hydroxylated in some or all of the chains. O-glycosylated on hydroxylated lysine residues. The O-linked glycan consists of a Glc-Gal disaccharide.
Function	Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name	Collagen Alpha-1(I Chain Alpha-1 Type I Collagen
Database Links	Reactome: R-HSA-114604 Reactome: R-HSA-1442490 Reactome: R-HSA-1474244 Reactome: R-HSA-1650814 Reactome: R-HSA-198933 Reactome: R-HSA-2022090 Reactome: R-HSA-202733 Reactome: R-HSA-216083 Reactome: R-HSA-2214320 Reactome: R-HSA-2243919 Reactome: R-HSA-3000170 Reactome: R-HSA-3000171 Reactome: R-HSA-3000178 Reactome: R-HSA-3000480 Reactome: R-HSA-430116 Reactome: R-HSA-75892 Reactome: R-HSA-76009 Reactome: R-HSA-8874081 Reactome: R-HSA-8940973 Reactome: R-HSA-8948216
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative CLIA Names	Collagen Alpha-1(I Chain CLIA kit Alpha-1 Type I Collagen CLIA kit COL1A1 CLIA kit
Specificity	This kit recognizes Human COL1α1 in samples. No significant cross-reactivity or interference between Human COL1α1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	96-109	103
EDTA plasma(n=8)	87-101	93
Cell culture media(n=8)	87-100	92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	193.69	471.48	1846.85	177.21	435.56	1730.77
Standard deviation	24.39	56.01	132.97	22.17	44.38	111.12
CV (%)	12.59	11.88	7.20	12.51	10.19	6.42

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human COL1A1 (Collagen alpha-1 (I) chain) CLIA Kit (STJC0000435)