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Human COL1A2 (Collagen alpha-2 (I) chain) CLIA Kit (STJC0000402)

Human COL1A2 (Collagen alpha-2 (I) chain) CLIA Kit (STJC0000402)
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  • 产品名称:Human COL1A2 (Collagen alpha-2 (I) chain) CLIA Kit (STJC0000402)
  • 产品型号:STJC0000402
  • 产品展商:ST john's laboratory
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简单介绍
Human COL1A2 (Collagen alpha-2 (I) chain) CLIA Kit (STJC0000402)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human COL1A2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: COL1A2
Gene ID: 1278
Uniprot ID: CO1A2_HUMAN
Specificity: This kit recognizes Human COL1 Alpha 2 in samples. No significant cross-reactivity or interference between Human COL1 Alpha 2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Forms the fibrils of tendon, ligaments and bones. In bones the fibrils are mineralized with calcium hydroxyapatite.
Post Translational Modifications Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.
Function Type I collagen is a member of group I collagen (fibrillar forming collagen).
Protein Name Collagen Alpha-2(I Chain
Alpha-2 Type I Collagen
Database Links Reactome: R-HSA-114604
Reactome: R-HSA-1442490
Reactome: R-HSA-1474244
Reactome: R-HSA-1650814
Reactome: R-HSA-198933
Reactome: R-HSA-2022090
Reactome: R-HSA-202733
Reactome: R-HSA-216083
Reactome: R-HSA-2173796
Reactome: R-HSA-2214320
Reactome: R-HSA-2243919
Reactome: R-HSA-3000170
Reactome: R-HSA-3000171
Reactome: R-HSA-3000178
Reactome: R-HSA-3000480
Reactome: R-HSA-430116
Reactome: R-HSA-6785807
Reactome: R-HSA-75892
Reactome: R-HSA-76009
Reactome: R-HSA-8874081
Reactome: R-HSA-8948216
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Collagen Alpha-2(I Chain CLIA kit
Alpha-2 Type I Collagen CLIA kit
COL1A2 CLIA kit
Specificity This kit recognizes Human COL1α2 in samples. No significant cross-reactivity or interference between Human COL1α2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-114 107
EDTA plasma(n=8) 86-96 91
Cell culture media(n=8) 89-101 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.56 189.05 753.17 109.77 198.00 744.85
Standard deviation 12.43 16.07 48.73 12.43 14.59 64.36
CV (%) 12.00 8.50 6.47 11.32 7.37 8.64
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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