Human EDN1 (Endothelin-1) Sandwich ELISA (STJE0001961) STJE0001961 上海玉博生物科技有限公司

Human EDN1 (Endothelin-1) Sandwich ELISA (STJE0001961)

简单介绍

Human EDN1 (Endothelin-1) Sandwich ELISA (STJE0001961)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.75pg/mL
Detection Limit:	1.25~80pg/mL
Short Description:	This EDN1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	EDN1
Gene ID:	1906
Uniprot ID:	EDN1_HUMAN
Specificity:	This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells. Also binds the DEAR/FBXW7-AS1 receptor.
Protein Name	Endothelin-1 Preproendothelin-1 Ppet1 Cleaved Into - Endothelin-1 Et-1 - Big Endothelin-1
Database Links	Reactome: R-HSA-375276 Reactome: R-HSA-416476
Cellular Localisation	Secreted
Alternative ELISA Names	Endothelin-1 ELISA kit Preproendothelin-1 ELISA kit Ppet1 Cleaved Into - Endothelin-1 ELISA kit Et-1 - Big Endothelin-1 ELISA kit EDN1 ELISA kit
Specificity	This kit recognizes Human ET-1 in samples. No significant cross-reactivity or interference between Human ET-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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