Human EFNA1 (Ephrin-A1) CLIA Kit (STJC0000439) STJC0000439 上海玉博生物科技有限公司

Human EFNA1 (Ephrin-A1) CLIA Kit (STJC0000439)

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产品名称：Human EFNA1 (Ephrin-A1) CLIA Kit (STJC0000439)
产品型号：STJC0000439
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human EFNA1 (Ephrin-A1) CLIA Kit (STJC0000439)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human EFNA1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	EFNA1
Gene ID:	1942
Uniprot ID:	EFNA1_HUMAN
Specificity:	This kit recognizes Human EFNA1 in samples. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at protein level).
Post Translational Modifications	Undergoes proteolysis by a metalloprotease to give rise to a soluble monomeric form. N-Glycosylation is required for binding to EPHA2 receptor and inducing its internalization.
Function	Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. Plays an important role in angiogenesis and tumor neovascularization. The recruitment of VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by down-regulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cone and regulates dendritic spine morphogenesis.
Protein Name	Ephrin-A1 Eph-Related Receptor Tyrosine Kinase Ligand 1 Lerk-1 Immediate Early Response Protein B61 Tumor Necrosis Factor Alpha-Induced Protein 4 Tnf Alpha-Induced Protein 4 Cleaved Into - Ephrin-A1 - Secreted Form
Database Links	Reactome: R-HSA-2682334 Reactome: R-HSA-3928663 Reactome: R-HSA-3928665
Cellular Localisation	Cell Membrane Lipid-Anchor Gpi-Anchor Ephrin-A1 Secreted Form: Secreted
Alternative CLIA Names	Ephrin-A1 CLIA kit Eph-Related Receptor Tyrosine Kinase Ligand 1 CLIA kit Lerk-1 CLIA kit Immediate Early Response Protein B61 CLIA kit Tumor Necrosis Factor Alpha-Induced Protein 4 CLIA kit Tnf Alpha-Induced Protein 4 Cleaved Into - Ephrin-A1 - Secreted Form CLIA kit EFNA1 CLIA kit EPLG1 CLIA kit LERK1 CLIA kit TNFAIP4 CLIA kit
Specificity	This kit recognizes Human EFNA1 in samples. No significant cross-reactivity or interference between Human EFNA1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	87-99	94
EDTA plasma(n=8)	90-104	96
Cell culture media(n=8)	90-103	97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	100.41	272.79	849.06	96.49	267.35	772.86
Standard deviation	8.77	22.31	55.27	8.80	30.26	54.56
CV (%)	8.73	8.18	6.51	9.12	11.32	7.06

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human EFNA1 (Ephrin-A1) CLIA Kit (STJC0000439)