Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958) STJE0001958 上海玉博生物科技有限公司

Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)

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产品名称：Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)
产品型号：STJE0001958
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	2.35pg/mL
Detection Limit:	3.91~250pg/mL
Short Description:	This EGF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	EGF
Gene ID:	1950
Uniprot ID:	EGF_HUMAN
Specificity:	This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	O-glycosylated with core 1-like and core 2-like glycans. It is uncertain if Ser-954 or Thr-955 is O-glycosylated. The modification here shows glycan heterogeneity: HexHexNAc (major) and Hex2HexNAc2 (minor).
Function	EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Can induce neurite outgrowth in motoneurons of the pond snail Lymnaea stagnalis in vitro.
Protein Name	Pro-Epidermal Growth Factor Egf Cleaved Into - Epidermal Growth Factor Urogastrone
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1227986 Reactome: R-HSA-1236382 Reactome: R-HSA-1236394 Reactome: R-HSA-1250196 Reactome: R-HSA-1251932 Reactome: R-HSA-1257604 Reactome: R-HSA-177929 Reactome: R-HSA-179812 Reactome: R-HSA-180292 Reactome: R-HSA-180336 Reactome: R-HSA-182971 Reactome: R-HSA-1963640 Reactome: R-HSA-1963642 Reactome: R-HSA-212718 Reactome: R-HSA-2219530 Reactome: R-HSA-5637810 Reactome: R-HSA-5638303 Reactome: R-HSA-5673001 Reactome: R-HSA-6785631 Reactome: R-HSA-6811558 Reactome: R-HSA-8847993 Reactome: R-HSA-8856825 Reactome: R-HSA-8856828 Reactome: R-HSA-8863795 Reactome: R-HSA-9009391 Reactome: R-HSA-9013507 Reactome: R-HSA-9634638 Reactome: R-HSA-9664565 Reactome: R-HSA-9665348 Reactome: R-HSA-9665686
Cellular Localisation	Membrane Single-Pass Type I Membrane Protein
Alternative ELISA Names	Pro-Epidermal Growth Factor ELISA kit Egf Cleaved Into - Epidermal Growth Factor ELISA kit Urogastrone ELISA kit EGF ELISA kit
Specificity	This kit recognizes Human EGF in samples. No significant cross-reactivity or interference between Human EGF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	85-100	92
EDTA plasma (n=8)	90-104	95
Cell culture media (n=8)	90-106	97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	13.20	24.10	114.10	13.00	25.80	123.20
Standard deviation	0.70	1.30	5.90	0.70	1.50	3.80
CV (%)	5.30	5.39	5.17	5.38	5.81	3.08

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human EGF (Pro-epidermal growth factor) Sandwich ELISA (STJE0001958)