Human ELN (Elastin) CLIA Kit (STJC0000544) STJC0000544 上海玉博生物科技有限公司

Human ELN (Elastin) CLIA Kit (STJC0000544)

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产品名称：Human ELN (Elastin) CLIA Kit (STJC0000544)
产品型号：STJC0000544
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human ELN (Elastin) CLIA Kit (STJC0000544)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	56.25ng/mL
Detection Limit:	93.75~6000ng/mL
Short Description:	This human ELN kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	ELN
Gene ID:	2006
Uniprot ID:	ELN_HUMAN
Specificity:	This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed within the outer myometrial smooth muscle and throughout the arteriolar tree of uterus (at protein level). Also expressed in the large arteries, lung and skin.
Post Translational Modifications	Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine. Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.
Function	Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle.
Protein Name	Elastin Tropoelastin
Database Links	Reactome: R-HSA-1474228 Reactome: R-HSA-1566948 Reactome: R-HSA-2129379
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Extracellular Matrix Of Elastic Fibers
Alternative CLIA Names	Elastin CLIA kit Tropoelastin CLIA kit ELN CLIA kit
Specificity	This kit recognizes Human ELN in samples. No significant cross-reactivity or interference between Human ELN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	89-104	96
EDTA plasma(n=8)	97-112	102
Cell culture media(n=8)	102-115	108

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	321.00	543.85	2645.01	339.13	561.97	2411.78
Standard deviation	34.44	43.29	204.46	36.56	47.99	252.03
CV (%)	10.73	7.96	7.73	10.78	8.54	10.45

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human ELN (Elastin) CLIA Kit (STJC0000544)