Human Ep-CAM (Epithelial Cell Adhesion Molecule) Sandwich ELISA (STJE0019889) STJE0019889 上海玉博生物科技有限公司

Human Ep-CAM (Epithelial Cell Adhesion Molecule) Sandwich ELISA (STJE0019889)

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产品名称：Human Ep-CAM (Epithelial Cell Adhesion Molecule) Sandwich ELISA (STJE0019889)
产品型号：STJE0019889
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human Ep-CAM (Epithelial Cell Adhesion Molecule) Sandwich ELISA (STJE0019889)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	1.12 pg/mL
Detection Limit:	3.13-200 pg/mL
Short Description:	This Ep-CAM Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	EPCAM
Gene ID:	4072
Uniprot ID:	EPCAM_HUMAN
Specificity:	This kit recognizes Human Ep-CAM in samples. No significant cross-reactivity or interference between Human Ep-CAM and analogues was observed.
Sample Type:	serum, plasma and other biological fluids

Tissue Specificity	Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post Translational Modifications	Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Protein Name	Epithelial Cell Adhesion Molecule Ep-Cam Adenocarcinoma-Associated Antigen Cell Surface Glycoprotein Trop-1 Epithelial Cell Surface Antigen Epithelial Glycoprotein Egp Epithelial Glycoprotein 314 Egp314 Hegp314 Ks 1/4 Antigen Ksa Major Gastrointestinal Tumor-Associated Protein Ga733-2 Tumor-Associated Calcium Signal Transducer 1 Cd Antigen Cd326
Database Links	Reactome: R-HSA-202733
Cellular Localisation	Lateral Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Tight Junction Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction
Alternative ELISA Names	Epithelial Cell Adhesion Molecule ELISA kit Ep-Cam ELISA kit Adenocarcinoma-Associated Antigen ELISA kit Cell Surface Glycoprotein Trop-1 ELISA kit Epithelial Cell Surface Antigen ELISA kit Epithelial Glycoprotein ELISA kit Egp ELISA kit Epithelial Glycoprotein 314 ELISA kit Egp314 ELISA kit Hegp314 ELISA kit Ks 1/4 Antigen ELISA kit Ksa ELISA kit Major Gastrointestinal Tumor-Associated Protein Ga733-2 ELISA kit Tumor-Associated Calcium Signal Transducer 1 ELISA kit Cd Antigen Cd326 ELISA kit EPCAM ELISA kit GA733-2 ELISA kit M1S2 ELISA kit M4S1 ELISA kit MIC18 ELISA kit TACSTD1 ELISA kit TROP1 ELISA kit
Specificity	This kit recognizes Human Ep-CAM in samples. No significant cross-reactivity or interference between Human Ep-CAM and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	94-108	100
EDTA plasma (n=8)	96-111	102
Cell culture media (n=8)	86-102	93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	10.70	27.38	77.08	10.75	27.27	76.60
Standard deviation	0.66	1.39	2.40	0.56	1.58	3.81
CV (%)	6.18	5.06	3.12	5.24	5.80	4.98

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human Ep-CAM (Epithelial Cell Adhesion Molecule) Sandwich ELISA (STJE0019889)