Human EPAS1 (Endothelial PAS domain-containing protein 1) Sandwich ELISA (STJE0003739) STJE0003739 上海玉博生物科技有限公司

Human EPAS1 (Endothelial PAS domain-containing protein 1) Sandwich ELISA (STJE0003739)

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产品名称：Human EPAS1 (Endothelial PAS domain-containing protein 1) Sandwich ELISA (STJE0003739)
产品型号：STJE0003739
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human EPAS1 (Endothelial PAS domain-containing protein 1) Sandwich ELISA (STJE0003739)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This EPAS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	EPAS1
Gene ID:	2034
Uniprot ID:	EPAS1_HUMAN
Specificity:	This kit recognizes Human HIF-2 Alpha in samples. No significant cross-reactivity or interference between Human HIF-2 Alpha and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization. In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation. Phosphorylated on multiple sites in the CTAD. The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains.
Function	Transcription factor involved in the induction of oxygen regulated genes. Heterodimerizes with ARNT.heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation requires recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX1 seems to activate CTAD.
Protein Name	Endothelial Pas Domain-Containing Protein 1 Epas-1 Basic-Helix-Loop-Helix-Pas Protein Mop2 Class E Basic Helix-Loop-Helix Protein 73 Bhlhe73 Hif-1-Alpha-Like Factor Hlf Hypoxia-Inducible Factor 2-Alpha Hif-2-Alpha Hif2-Alpha Member Of Pas Protein 2 Pas Domain-Containing Protein 2
Database Links	Reactome: R-HSA-1234158 Reactome: R-HSA-1234174 Reactome: R-HSA-1234176 Reactome: R-HSA-452723 Reactome: R-HSA-8849473 Reactome: R-HSA-8951664 Reactome: R-HSA-9664873
Cellular Localisation	Nucleus Nucleus Speckle Colocalizes With Hif3a In The Nucleus And Speckles
Alternative ELISA Names	Endothelial Pas Domain-Containing Protein 1 ELISA kit Epas-1 ELISA kit Basic-Helix-Loop-Helix-Pas Protein Mop2 ELISA kit Class E Basic Helix-Loop-Helix Protein 73 ELISA kit Bhlhe73 ELISA kit Hif-1-Alpha-Like Factor ELISA kit Hlf ELISA kit Hypoxia-Inducible Factor 2-Alpha ELISA kit Hif-2-Alpha ELISA kit Hif2-Alpha ELISA kit Member Of Pas Protein 2 ELISA kit Pas Domain-Containing Protein 2 ELISA kit EPAS1 ELISA kit BHLHE73 ELISA kit HIF2A ELISA kit MOP2 ELISA kit PASD2 ELISA kit
Specificity	This kit recognizes Human HIF-2α in samples. No significant cross-reactivity or interference between Human HIF-2α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	95-110	101
EDTA plasma (n=8)	94-112	102
Cell culture media (n=8)	88-102	93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.48	1.59	3.82	0.45	1.72	3.58
Standard deviation	0.02	0.08	0.20	0.02	0.10	0.16
CV (%)	4.17	5.03	5.24	4.44	5.81	4.47

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human EPAS1 (Endothelial PAS domain-containing protein 1) Sandwich ELISA (STJE0003739)