Human EPCAM (Epithelial cell adhesion molecule) CLIA Kit (STJC0000819) STJC0000819 上海玉博生物科技有限公司

Human EPCAM (Epithelial cell adhesion molecule) CLIA Kit (STJC0000819)

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产品名称：Human EPCAM (Epithelial cell adhesion molecule) CLIA Kit (STJC0000819)
产品型号：STJC0000819
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human EPCAM (Epithelial cell adhesion molecule) CLIA Kit (STJC0000819)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.19IU/mL
Detection Limit:	0.31~20IU/mL
Short Description:	This human EPCAM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	EPCAM
Gene ID:	4072
Uniprot ID:	EPCAM_HUMAN
Specificity:	This kit recognizes Human Ep-CAM/CD326 in samples. No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC). Levels rapidly diminish as soon as ESC's differentiate (at protein levels). Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma.
Post Translational Modifications	Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability.
Function	May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
Protein Name	Epithelial Cell Adhesion Molecule Ep-Cam Adenocarcinoma-Associated Antigen Cell Surface Glycoprotein Trop-1 Epithelial Cell Surface Antigen Epithelial Glycoprotein Egp Epithelial Glycoprotein 314 Egp314 Hegp314 Ks 1/4 Antigen Ksa Major Gastrointestinal Tumor-Associated Protein Ga733-2 Tumor-Associated Calcium Signal Transducer 1 Cd Antigen Cd326
Database Links	Reactome: R-HSA-202733
Cellular Localisation	Lateral Cell Membrane Single-Pass Type I Membrane Protein Cell Junction Tight Junction Colocalizes With Cldn7 At The Lateral Cell Membrane And Tight Junction
Alternative CLIA Names	Epithelial Cell Adhesion Molecule CLIA kit Ep-Cam CLIA kit Adenocarcinoma-Associated Antigen CLIA kit Cell Surface Glycoprotein Trop-1 CLIA kit Epithelial Cell Surface Antigen CLIA kit Epithelial Glycoprotein CLIA kit Egp CLIA kit Epithelial Glycoprotein 314 CLIA kit Egp314 CLIA kit Hegp314 CLIA kit Ks 1/4 Antigen CLIA kit Ksa CLIA kit Major Gastrointestinal Tumor-Associated Protein Ga733-2 CLIA kit Tumor-Associated Calcium Signal Transducer 1 CLIA kit Cd Antigen Cd326 CLIA kit EPCAM CLIA kit GA733-2 CLIA kit M1S2 CLIA kit M4S1 CLIA kit MIC18 CLIA kit TACSTD1 CLIA kit TROP1 CLIA kit
Specificity	This kit recognizes Human Ep-CAM/CD326 in samples. No significant cross-reactivity or interference between Human Ep-CAM/CD326 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-107	100
EDTA plasma(n=8)	87-97	92
Cell culture media(n=8)	96-114	104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (IU/mL)	0.91	1.91	7.30	0.86	1.75	7.13
Standard deviation	0.09	0.22	0.75	0.07	0.14	0.49
CV (%)	9.89	11.52	10.27	8.14	8.00	6.87

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human EPCAM (Epithelial cell adhesion molecule) CLIA Kit (STJC0000819)