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Human CCL3 (C-C motif chemokine 3) CLIA Kit (STJC0000021)

Human CCL3 (C-C motif chemokine 3) CLIA Kit (STJC0000021)
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  • 产品名称:Human CCL3 (C-C motif chemokine 3) CLIA Kit (STJC0000021)
  • 产品型号:STJC0000021
  • 产品展商:ST john's laboratory
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简单介绍
Human CCL3 (C-C motif chemokine 3) CLIA Kit (STJC0000021)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human CCL3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: CCL3
Gene ID: 6348
Uniprot ID: CCL3_HUMAN
Specificity: This kit recognizes Human MIP-1 Alpha in samples. No significant cross-reactivity or interference between Human MIP-1 Alpha and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Protein Name C-C Motif Chemokine 3
G0/G1 Switch Regulatory Protein 19-1
Macrophage Inflammatory Protein 1-Alpha
Mip-1-Alpha
Pat 464.1
Sis-Beta
Small-Inducible Cytokine A3
Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69
Ld78-Alpha(4-69
Database Links Reactome: R-HSA-380108
Reactome: R-HSA-6783783
Cellular Localisation Secreted
Alternative CLIA Names C-C Motif Chemokine 3 CLIA kit
G0/G1 Switch Regulatory Protein 19-1 CLIA kit
Macrophage Inflammatory Protein 1-Alpha CLIA kit
Mip-1-Alpha CLIA kit
Pat 464.1 CLIA kit
Sis-Beta CLIA kit
Small-Inducible Cytokine A3 CLIA kit
Tonsillar Lymphocyte Ld78 Alpha Protein Cleaved Into - Mip-1-Alpha(4-69 CLIA kit
Ld78-Alpha(4-69 CLIA kit
CCL3 CLIA kit
G0S19-1 CLIA kit
MIP1A CLIA kit
SCYA3 CLIA kit
Specificity This kit recognizes Human MIP-1α in samples. No significant cross-reactivity or interference between Human MIP-1α and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-98 93
EDTA plasma(n=8) 97-111 104
Cell culture media(n=8) 100-114 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 39.20 107.86 347.92 39.81 115.66 358.19
Standard deviation 3.39 9.71 31.52 4.94 13.61 24.29
CV (%) 8.65 9.00 9.06 12.41 11.77 6.78
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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