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Human F8 (Coagulation factor VIII) Sandwich ELISA (STJE0004128)

Human F8 (Coagulation factor VIII) Sandwich ELISA (STJE0004128)
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  • 产品名称:Human F8 (Coagulation factor VIII) Sandwich ELISA (STJE0004128)
  • 产品型号:STJE0004128
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human F8 (Coagulation factor VIII) Sandwich ELISA (STJE0004128)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.1IU/mL
Detection Limit: 0.16~10IU/mL
Short Description: This F8 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: F8
Gene ID: 2157
Uniprot ID: FA8_HUMAN
Specificity: This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for F9/factor IXa when it converts F10/factor X to the activated form, factor Xa.
Protein Name Coagulation Factor Viii
Antihemophilic Factor
Ahf
Procoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain
Database Links Reactome: R-HSA-114608
Reactome: R-HSA-140837
Reactome: R-HSA-140875
Reactome: R-HSA-163841
Reactome: R-HSA-204005
Reactome: R-HSA-5694530
Reactome: R-HSA-9672383
Reactome: R-HSA-9672387
Reactome: R-HSA-9672391
Reactome: R-HSA-9672393
Reactome: R-HSA-9672395
Reactome: R-HSA-9672396
Reactome: R-HSA-9672397
Reactome: R-HSA-9673202
Reactome: R-HSA-9674519
Cellular Localisation Secreted
Extracellular Space
Alternative ELISA Names Coagulation Factor Viii ELISA kit
Antihemophilic Factor ELISA kit
Ahf ELISA kit
Procoagulant Component Cleaved Into - Factor Viiia Heavy Chain - 200 Kda Isoform - Factor Viiia Heavy Chain - 92 Kda Isoform - Factor Viii B Chain - Factor Viiia Light Chain ELISA kit
F8 ELISA kit
F8C ELISA kit
Specificity This kit recognizes Human F8 in samples. No significant cross-reactivity or interference between Human F8 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 99-109 102
EDTA plasma (n=8) 87-96 90
Cell culture media (n=8) 96-108 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (IU/mL) 0.44 1.19 4.01 0.49 1.02 4.03
Standard deviation 0.02 0.06 0.17 0.03 0.05 0.21
CV (%) 4.55 5.04 4.24 6.12 4.90 5.21
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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