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Human FABP4 (Fatty acid-binding protein, adipocyte) Sandwich ELISA (STJE0002106)

Human FABP4 (Fatty acid-binding protein, adipocyte) Sandwich ELISA (STJE0002106)
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  • 产品名称:Human FABP4 (Fatty acid-binding protein, adipocyte) Sandwich ELISA (STJE0002106)
  • 产品型号:STJE0002106
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human FABP4 (Fatty acid-binding protein, adipocyte) Sandwich ELISA (STJE0002106)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.23ng/mL
Detection Limit: 0.39~25ng/mL
Short Description: This FABP4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FABP4
Gene ID: 2167
Uniprot ID: FABP4_HUMAN
Specificity: This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Fatty Acid-Binding Protein - Adipocyte
Adipocyte Lipid-Binding Protein
Albp
Adipocyte-Type Fatty Acid-Binding Protein
A-Fabp
Afabp
Fatty Acid-Binding Protein 4
Database Links Reactome: R-HSA-163560
Reactome: R-HSA-381340
Cellular Localisation Cytoplasm
Nucleus
Depending On The Nature Of The Ligand
A Conformation Change Exposes A Nuclear Localization Motif And The Protein Is Transported Into The Nucleus
Subject To Constitutive Nuclear Export
Alternative ELISA Names Fatty Acid-Binding Protein - Adipocyte ELISA kit
Adipocyte Lipid-Binding Protein ELISA kit
Albp ELISA kit
Adipocyte-Type Fatty Acid-Binding Protein ELISA kit
A-Fabp ELISA kit
Afabp ELISA kit
Fatty Acid-Binding Protein 4 ELISA kit
FABP4 ELISA kit
Specificity This kit recognizes Human FABP4 in samples. No significant cross-reactivity or interference between Human FABP4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-105 99
EDTA plasma (n=8) 92-104 99
Cell culture media (n=8) 84-98 90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.23 2.72 9.72 1.20 2.74 9.02
Standard deviation 0.07 0.13 0.39 0.07 0.12 0.32
CV (%) 5.69 4.78 4.01 5.83 4.38 3.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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