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Human FGF18 (Fibroblast growth factor 18) Sandwich ELISA (STJE0003839)

Human FGF18 (Fibroblast growth factor 18) Sandwich ELISA (STJE0003839)
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  • 产品名称:Human FGF18 (Fibroblast growth factor 18) Sandwich ELISA (STJE0003839)
  • 产品型号:STJE0003839
  • 产品展商:ST john's laboratory
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简单介绍
Human FGF18 (Fibroblast growth factor 18) Sandwich ELISA (STJE0003839)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This FGF18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: FGF18
Gene ID: 8817
Uniprot ID: FGF18_HUMAN
Specificity: This kit recognizes Human FGF18 in samples. No significant cross-reactivity or interference between Human FGF18 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Fibroblast Growth Factor 18
Fgf-18
Zfgf5
Database Links Reactome: R-HSA-109704
Reactome: R-HSA-1257604
Reactome: R-HSA-1839130
Reactome: R-HSA-190322
Reactome: R-HSA-190371
Reactome: R-HSA-190372
Reactome: R-HSA-190375
Reactome: R-HSA-2033519
Reactome: R-HSA-2219530
Reactome: R-HSA-5654221
Reactome: R-HSA-5654227
Reactome: R-HSA-5654228
Reactome: R-HSA-5654695
Reactome: R-HSA-5654699
Reactome: R-HSA-5654700
Reactome: R-HSA-5654704
Reactome: R-HSA-5654706
Reactome: R-HSA-5654710
Reactome: R-HSA-5654712
Reactome: R-HSA-5654719
Reactome: R-HSA-5654720
Reactome: R-HSA-5654727
Reactome: R-HSA-5654732
Reactome: R-HSA-5654733
Reactome: R-HSA-5655253
Reactome: R-HSA-5655332
Reactome: R-HSA-5658623
Reactome: R-HSA-5673001
Reactome: R-HSA-6811558
Cellular Localisation Secreted
Alternative ELISA Names Fibroblast Growth Factor 18 ELISA kit
Fgf-18 ELISA kit
Zfgf5 ELISA kit
FGF18 ELISA kit
UNQ420 ELISA kit
PRO856 ELISA kit
Specificity This kit recognizes Human FGF18 in samples. No significant cross-reactivity or interference between Human FGF18 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 91
EDTA plasma (n=8) 97-107 102
Cell culture media (n=8) 94-108 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 45.84 110.71 346.06 49.18 102.01 354.77
Standard deviation 2.98 5.17 18.55 3.14 5.80 17.31
CV (%) 6.50 4.67 5.36 6.38 5.69 4.88
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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