Human FLOT2 (Flotillin-2) CLIA Kit (STJC0000232) STJC0000232 上海玉博生物科技有限公司

Human FLOT2 (Flotillin-2) CLIA Kit (STJC0000232)

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产品名称：Human FLOT2 (Flotillin-2) CLIA Kit (STJC0000232)
产品型号：STJC0000232
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human FLOT2 (Flotillin-2) CLIA Kit (STJC0000232)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This human FLOT2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	FLOT2
Gene ID:	2319
Uniprot ID:	FLOT2_HUMAN
Specificity:	This kit recognizes Human FLOT2 in samples. No significant cross-reactivity or interference between Human FLOT2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	In skin, expressed in epidermis and epidermal appendages but not in dermis. Expressed in all layers of the epidermis except the basal layer. In hair follicles, expressed in the suprabasal layer but not the basal layer. Also expressed in melanoma and carcinoma cell lines, fibroblasts and foreskin melanocytes.
Post Translational Modifications	ZDHHC5-catalyzed palmitoylation predominantly occurs at Cys-4. ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.
Function	May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. May be involved in epidermal cell adhesion and epidermal structure and function.
Protein Name	Flotillin-2 Epidermal Surface Antigen Esa Membrane Component Chromosome 17 Surface Marker 1
Database Links	Reactome: R-HSA-5213460 Reactome: R-HSA-5675482 Reactome: R-HSA-8849932 Reactome: R-HSA-8980692 Reactome: R-HSA-9013026 Reactome: R-HSA-9013106 Reactome: R-HSA-9696264 Reactome: R-HSA-9696273
Cellular Localisation	Cell Membrane Peripheral Membrane Protein Membrane Caveola Endosome Lipid-Anchor Membrane-Associated Protein Of Caveolae
Alternative CLIA Names	Flotillin-2 CLIA kit Epidermal Surface Antigen CLIA kit Esa CLIA kit Membrane Component Chromosome 17 Surface Marker 1 CLIA kit FLOT2 CLIA kit ESA1 CLIA kit M17S1 CLIA kit
Specificity	This kit recognizes Human FLOT2 in samples. No significant cross-reactivity or interference between Human FLOT2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	97-112	103
EDTA plasma(n=8)	96-110	104
Cell culture media(n=8)	100-115	106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.49	0.87	4.26	0.45	0.84	3.95
Standard deviation	0.04	0.10	0.39	0.04	0.09	0.38
CV (%)	8.16	11.49	9.15	8.89	10.71	9.62

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human FLOT2 (Flotillin-2) CLIA Kit (STJC0000232)