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Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit (STJC0000794)

Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit (STJC0000794)
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  • 产品名称:Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit (STJC0000794)
  • 产品型号:STJC0000794
  • 产品展商:ST john's laboratory
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简单介绍
Human G6PD (Glucose-6-phosphate 1-dehydrogenase) CLIA Kit (STJC0000794)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human G6PD kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: G6PD
Gene ID: 2539
Uniprot ID: G6PD_HUMAN
Specificity: This kit recognizes Human G6PD in samples. No significant cross-reactivity or interference between Human G6PD and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Isoform Long is found in lymphoblasts, granulocytes and sperm.
Post Translational Modifications Acetylated by ELP3 at Lys-403.acetylation inhibits its homodimerization and enzyme activity. Deacetylated by SIRT2 at Lys-403.deacetylation stimulates its enzyme activity.
Function Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis.
Protein Name Glucose-6-Phosphate 1-Dehydrogenase
G6pd
Database Links Reactome: R-HSA-5628897
Reactome: R-HSA-71336
Reactome: R-HSA-9818028
Cellular Localisation Cytoplasm
Cytosol
Membrane
Peripheral Membrane Protein
Alternative CLIA Names Glucose-6-Phosphate 1-Dehydrogenase CLIA kit
G6pd CLIA kit
G6PD CLIA kit
Specificity This kit recognizes Human G6PD in samples. No significant cross-reactivity or interference between Human G6PD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-104 95
EDTA plasma(n=8) 97-109 103
Cell culture media(n=8) 96-110 102

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 206.49 361.06 1587.89 209.52 373.10 1659.47
Standard deviation 20.19 38.53 140.69 26.97 32.68 124.13
CV (%) 9.78 10.67 8.86 12.87 8.76 7.48
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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