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Human IL1RN (Interleukin-1 receptor antagonist protein) Sandwich ELISA (STJE0001980)

Human IL1RN (Interleukin-1 receptor antagonist protein) Sandwich ELISA (STJE0001980)
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  • 产品名称:Human IL1RN (Interleukin-1 receptor antagonist protein) Sandwich ELISA (STJE0001980)
  • 产品型号:STJE0001980
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human IL1RN (Interleukin-1 receptor antagonist protein) Sandwich ELISA (STJE0001980)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This IL1RN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL1RN
Gene ID: 3557
Uniprot ID: IL1RA_HUMAN
Specificity: This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Anti-inflammatory antagonist of interleukin-1 family of proinflammatory cytokines such as interleukin-1beta/IL1B and interleukin-1alpha/IL1A. Protects from immune dysregulation and uncontrolled systemic inflammation triggered by IL1 for a range of innate stimulatory agents such as pathogens.
Protein Name Interleukin-1 Receptor Antagonist Protein
Il-1rn
Il-1ra
Irap
Icil-1ra
Il1 Inhibitor
Anakinra
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-9020702
Cellular Localisation Isoform 1: Secreted
Isoform 2: Cytoplasm
Isoform 3: Cytoplasm
Isoform 4: Cytoplasm
Alternative ELISA Names Interleukin-1 Receptor Antagonist Protein ELISA kit
Il-1rn ELISA kit
Il-1ra ELISA kit
Irap ELISA kit
Icil-1ra ELISA kit
Il1 Inhibitor ELISA kit
Anakinra ELISA kit
IL1RN ELISA kit
IL1F3 ELISA kit
IL1RA ELISA kit
Specificity This kit recognizes Human IL-1ra/IL-1F3 in samples. No significant cross-reactivity or interference between Human IL-1ra/IL-1F3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-110 101
EDTA plasma (n=8) 92-108 99
Cell culture media (n=8) 88-101 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 96.60 253.23 775.71 104.45 248.93 801.14
Standard deviation 5.12 13.12 41.11 5.89 12.92 43.58
CV (%) 5.30 5.18 5.30 5.64 5.19 5.44
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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