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Human GPC4 (Glypican-4) CLIA Kit (STJC0000759)

Human GPC4 (Glypican-4) CLIA Kit (STJC0000759)
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  • 产品名称:Human GPC4 (Glypican-4) CLIA Kit (STJC0000759)
  • 产品型号:STJC0000759
  • 产品展商:ST john's laboratory
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简单介绍
Human GPC4 (Glypican-4) CLIA Kit (STJC0000759)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human GPC4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: GPC4
Gene ID: 2239
Uniprot ID: GPC4_HUMAN
Specificity: This kit recognizes Human GPC4 in samples. No significant cross-reactivity or interference between Human GPC4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system.
Protein Name Glypican-4
K-Glypican Cleaved Into - Secreted Glypican-4
Database Links Reactome: R-HSA-1971475
Reactome: R-HSA-2022928
Reactome: R-HSA-2024096
Reactome: R-HSA-3560783
Reactome: R-HSA-3560801
Reactome: R-HSA-3656237
Reactome: R-HSA-3656253
Reactome: R-HSA-4420332
Reactome: R-HSA-9694614
Reactome: R-HSA-975634
Cellular Localisation Cell Membrane
Lipid-Anchor
Gpi-Anchor
Extracellular Side
Secreted Glypican-4: Secreted
Extracellular Space
Alternative CLIA Names Glypican-4 CLIA kit
K-Glypican Cleaved Into - Secreted Glypican-4 CLIA kit
GPC4 CLIA kit
UNQ474 CLIA kit
PRO937 CLIA kit
Specificity This kit recognizes Human GPC4 in samples. No significant cross-reactivity or interference between Human GPC4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-104 96
EDTA plasma(n=8) 93-107 98
Cell culture media(n=8) 91-107 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 103.46 200.33 888.70 94.98 188.87 832.81
Standard deviation 10.50 19.75 101.93 10.54 17.98 71.21
CV (%) 10.15 9.86 11.47 11.10 9.52 8.55
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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