Human GRN (Progranulin) Sandwich ELISA (STJE0003076) STJE0003076 上海玉博生物科技有限公司

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产品名称：Human GRN (Progranulin) Sandwich ELISA (STJE0003076)
产品型号：STJE0003076
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human GRN (Progranulin) Sandwich ELISA (STJE0003076)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This GRN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	GRN
Gene ID:	2896
Uniprot ID:	GRN_HUMAN
Specificity:	This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Cleaved by ELANE.proteolysis is blocked by SLPI and is concentration- and time-dependent and induces CXCL8/IL-8 production.granulin-3 and granulin-4 are resistant to ELANE. Cleaved by CTSL in lysosome thus regulating the maturation and turnover of progranulin within the lysosome.
Function	Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation. Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes. Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB. In addition, functions as wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures. Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases. Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity. Granulin-4: Promotes proliferation of the epithelial cell line A431 in culture. Granulin-3: Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8. Granulin-7: Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
Protein Name	Progranulin Pgrn Acrogranin Epithelin Precursor Glycoprotein Of 88 Kda Gp88 Glycoprotein 88 Granulin Precursor Pc Cell-Derived Growth Factor Pcdgf Proepithelin Pepi Cleaved Into - Paragranulin - Granulin-1 Granulin G - Granulin-2 Granulin F - Granulin-3 Epithelin-2 Granulin B - Granulin-4 Epithelin-1 Granulin A - Granulin-5 Granulin C - Granulin-6 Granulin D - Granulin-7 Granulin E
Database Links	Reactome: R-HSA-6798695
Cellular Localisation	Secreted Lysosome Endocytosed By Sort1 And Delivred To Lysosomes Targeted To Lysosome By Psap Via M6pr And Lrp1 In Both Biosynthetic And Endocytic Pathways Co-Localized With Gba1 In The Intracellular Trafficking Compartments Until To Lysosome
Alternative ELISA Names	Progranulin ELISA kit Pgrn ELISA kit Acrogranin ELISA kit Epithelin Precursor ELISA kit Glycoprotein Of 88 Kda ELISA kit Gp88 ELISA kit Glycoprotein 88 ELISA kit Granulin Precursor ELISA kit Pc Cell-Derived Growth Factor ELISA kit Pcdgf ELISA kit Proepithelin ELISA kit Pepi Cleaved Into - Paragranulin - Granulin-1 ELISA kit Granulin G - Granulin-2 ELISA kit Granulin F - Granulin-3 ELISA kit Epithelin-2 ELISA kit Granulin B - Granulin-4 ELISA kit Epithelin-1 ELISA kit Granulin A - Granulin-5 ELISA kit Granulin C - Granulin-6 ELISA kit Granulin D - Granulin-7 ELISA kit Granulin E ELISA kit GRN ELISA kit
Specificity	This kit recognizes Human PGRN in samples. No significant cross-reactivity or interference between Human PGRN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	94-111	102
EDTA plasma (n=8)	85-98	91
Cell culture media (n=8)	85-98	92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	206.38	339.77	1769.00	224.98	330.28	1882.28
Standard deviation	11.74	15.73	83.14	12.82	18.99	64.37
CV (%)	5.69	4.63	4.70	5.70	5.75	3.42

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human GRN (Progranulin) Sandwich ELISA (STJE0003076)