Human GSTA1 (Glutathione S-transferase A1) Sandwich ELISA (STJE0004082) STJE0004082 上海玉博生物科技有限公司

Human GSTA1 (Glutathione S-transferase A1) Sandwich ELISA (STJE0004082)

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产品名称：Human GSTA1 (Glutathione S-transferase A1) Sandwich ELISA (STJE0004082)
产品型号：STJE0004082
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human GSTA1 (Glutathione S-transferase A1) Sandwich ELISA (STJE0004082)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.19ng/mL
Detection Limit:	0.31~20ng/mL
Short Description:	This GSTA1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	GSTA1
Gene ID:	2938
Uniprot ID:	GSTA1_HUMAN
Specificity:	This kit recognizes Human GST Alpha 1 in samples. No significant cross-reactivity or interference between Human GST Alpha 1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Glutathione S-transferase that catalyzes the nucleophilic attack of the sulfur atom of glutathione on the electrophilic groups of a wide range of exogenous and endogenous compounds (Probable). Involved in the formation of glutathione conjugates of both prostaglandin A2 (PGA2) and prostaglandin J2 (PGJ2). It also catalyzes the isomerization of D5-androstene-3,17-dione (AD) into D4-androstene-3,17-dione and may therefore play an important role in hormone biosynthesis. Through its glutathione-dependent peroxidase activity toward the fatty acid hydroperoxide (13S)-hydroperoxy-(9Z,11E)-octadecadienoate/13-HPODE it is also involved in the metabolism of oxidized linoleic acid.
Protein Name	Glutathione S-Transferase A1 13-Hydroperoxyoctadecadienoate Peroxidase Androst-5-Ene-3 -17-Dione Isomerase Gst Ha Subunit 1 Gst Class-Alpha Member 1 Gst-Epsilon Gsta1-1 Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed
Database Links	Reactome: R-HSA-156590 Reactome: R-HSA-189483 Reactome: R-HSA-9748787 Reactome: R-HSA-9818027
Cellular Localisation	Cytoplasm
Alternative ELISA Names	Glutathione S-Transferase A1 ELISA kit 13-Hydroperoxyoctadecadienoate Peroxidase ELISA kit Androst-5-Ene-3 -17-Dione Isomerase ELISA kit Gst Ha Subunit 1 ELISA kit Gst Class-Alpha Member 1 ELISA kit Gst-Epsilon ELISA kit Gsta1-1 ELISA kit Gth1 Cleaved Into - Glutathione S-Transferase A1 - N-Terminally Processed ELISA kit GSTA1 ELISA kit
Specificity	This kit recognizes Human GSTα1 in samples. No significant cross-reactivity or interference between Human GSTα1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	100-111	103
EDTA plasma (n=8)	89-101	94
Cell culture media (n=8)	101-111	104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.86	2.28	8.66	0.90	2.00	8.79
Standard deviation	0.05	0.11	0.44	0.05	0.09	0.35
CV (%)	5.81	4.82	5.08	5.56	4.50	3.98

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human GSTA1 (Glutathione S-transferase A1) Sandwich ELISA (STJE0004082)