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Human IL-35 (Interleukin 35) Sandwich ELISA (STJE0003718)

Human IL-35 (Interleukin 35) Sandwich ELISA (STJE0003718)
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  • 产品名称:Human IL-35 (Interleukin 35) Sandwich ELISA (STJE0003718)
  • 产品型号:STJE0003718
  • 产品展商:ST john's laboratory
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简单介绍
Human IL-35 (Interleukin 35) Sandwich ELISA (STJE0003718)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This IL-35 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL12A
EBI3
Gene ID: 3592
10148
Uniprot ID: IL12A_HUMAN
IL27B_HUMAN
Specificity: This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Alternative ELISA Names Interleukin-12 Subunit Alpha ELISA kit
Il-12a ELISA kit
Cytotoxic Lymphocyte Maturation Factor 35 Kda Subunit ELISA kit
Clmf P35 ELISA kit
Il-12 Subunit P35 ELISA kit
Nk Cell Stimulatory Factor Chain 1 ELISA kit
Nksf1 ELISA kit
IL12A ELISA kit
NKSF1 ELISA kit
Interleukin-27 Subunit Beta ELISA kit
Il-27 Subunit Beta ELISA kit
Il-27b ELISA kit
Epstein-Barr Virus-Induced Gene 3 Protein ELISA kit
Ebv-Induced Gene 3 Protein ELISA kit
EBI3 ELISA kit
IL27B ELISA kit
Specificity This kit recognizes Human IL-35 in samples. No significant cross-reactivity or interference between Human IL-35 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 93
EDTA plasma (n=8) 95-108 101
Cell culture media (n=8) 95-108 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 52.85 89.54 415.10 52.95 94.65 452.88
Standard deviation 3.28 4.59 15.28 3.49 5.36 15.49
CV (%) 6.21 5.13 3.68 6.59 5.66 3.42
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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