Human IL-6 (Interleukin 10) Sandwich ELISA (STJE0019956) STJE0019956 上海玉博生物科技有限公司

Human IL-6 (Interleukin 10) Sandwich ELISA (STJE0019956)

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产品名称：Human IL-6 (Interleukin 10) Sandwich ELISA (STJE0019956)
产品型号：STJE0019956
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human IL-6 (Interleukin 10) Sandwich ELISA (STJE0019956)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.49 pg/mL
Detection Limit:	0.78-50 pg/mL
Short Description:	This IL-6 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	IL10
Gene ID:	3586
Uniprot ID:	IL10_HUMAN
Specificity:	This kit recognizes Human IL-10 in samples.No significant cross-reactivity or interference between Human IL-10 and analogues was observed
Sample Type:	serum, plasma and other biological fluids

Tissue Specificity	Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types.
Function	Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation. In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling.
Protein Name	Interleukin-10 Il-10 Cytokine Synthesis Inhibitory Factor Csif
Database Links	Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-8950505 Reactome: R-HSA-9662834 Reactome: R-HSA-9664323
Cellular Localisation	Secreted
Alternative ELISA Names	Interleukin-10 ELISA kit Il-10 ELISA kit Cytokine Synthesis Inhibitory Factor ELISA kit Csif ELISA kit IL10 ELISA kit
Specificity	This kit recognizes Human IL-10 in samples. No significant cross-reactivity or interference between Human IL-10 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	85-98	92
EDTA plasma (n=8)	94-110	101
Cell culture media (n=8)	94-107	101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	2.77	4.57	23.31	2.90	4.43	25.03
Standard deviation	0.14	0.22	0.75	0.19	0.26	1.18
CV (%)	5.09	4.75	3.21	6.68	5.79	4.70

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human IL-6 (Interleukin 10) Sandwich ELISA (STJE0019956)