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Human IL24 (Interleukin-24) Sandwich ELISA (STJE0003704)

Human IL24 (Interleukin-24) Sandwich ELISA (STJE0003704)
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  • 产品名称:Human IL24 (Interleukin-24) Sandwich ELISA (STJE0003704)
  • 产品型号:STJE0003704
  • 产品展商:ST john's laboratory
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简单介绍
Human IL24 (Interleukin-24) Sandwich ELISA (STJE0003704)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This IL24 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL24
Gene ID: 11009
Uniprot ID: IL24_HUMAN
Specificity: This kit recognizes Human IL-24 in samples. No significant cross-reactivity or interference between Human IL-24 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Glycosylated. Ubiquitination at Lys-122 promotes proteasomal degradation.
Function Multifunctional cytokine mainly produced by T-cells that plays a regulatory role in immune response, tissue homeostasis, host defense, and oncogenesis. Possesses antiviral functions and induces the type I intereferon response during influenza infection. Signals through two receptor complexes IL20RA/IL20RB or IL20RB/IL22RA1. In turn, stimulates the JAK1-STAT3 and MAPK pathways and promotes the secretion of pro-inflammatory mediators including IL8 and MMP1. Intracellularly, maintains endoplasmic reticulum homeostasis by restricting the eIF2alpha-CHOP pathway-mediated stress signal. In addition, acts as a quality control mechanism for the ubiquitin proteasome system by alerting the cell to proteasome dysfunction through activation of PKR/EIF2AK2.
Protein Name Interleukin-24
Il-24
Melanoma Differentiation-Associated Gene 7 Protein
Mda-7
Suppression Of Tumorigenicity 16 Protein
Database Links Reactome: R-HSA-8854691
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-24 ELISA kit
Il-24 ELISA kit
Melanoma Differentiation-Associated Gene 7 Protein ELISA kit
Mda-7 ELISA kit
Suppression Of Tumorigenicity 16 Protein ELISA kit
IL24 ELISA kit
MDA7 ELISA kit
ST16 ELISA kit
Specificity This kit recognizes Human IL-24 in samples. No significant cross-reactivity or interference between Human IL-24 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 96
EDTA plasma (n=8) 90-108 98
Cell culture media (n=8) 89-102 95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 49.20 89.40 433.80 52.80 93.00 421.90
Standard deviation 3.30 4.90 18.20 2.70 4.20 20.30
CV (%) 6.71 5.48 4.20 5.11 4.52 4.81
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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