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Human IL34 (Interleukin-34) Sandwich ELISA (STJE0003131)

Human IL34 (Interleukin-34) Sandwich ELISA (STJE0003131)
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  • 产品名称:Human IL34 (Interleukin-34) Sandwich ELISA (STJE0003131)
  • 产品型号:STJE0003131
  • 产品展商:ST john's laboratory
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简单介绍
Human IL34 (Interleukin-34) Sandwich ELISA (STJE0003131)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 56.25pg/mL
Detection Limit: 93.75~6000pg/mL
Short Description: This IL34 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: IL34
Gene ID: 146433
Uniprot ID: IL34_HUMAN
Specificity: This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
Protein Name Interleukin-34
Il-34
Database Links Reactome: R-HSA-449836
Reactome: R-HSA-9680350
Cellular Localisation Secreted
Alternative ELISA Names Interleukin-34 ELISA kit
Il-34 ELISA kit
IL34 ELISA kit
C16orf77 ELISA kit
Specificity This kit recognizes Human IL-34 in samples. No significant cross-reactivity or interference between Human IL-34 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-102 97
EDTA plasma (n=8) 84-97 91
Cell culture media (n=8) 92-106 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 292.00 719.71 2331.63 295.64 706.23 2493.50
Standard deviation 15.07 38.22 90.47 15.46 39.62 112.71
CV (%) 5.16 5.31 3.88 5.23 5.61 4.52
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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