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Human iPLA2 (Phospholipase A2, Calcium Independent) Sandwich ELISA Kit Ready-To-Use (STJE0016426)
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Human iPLA2 (Phospholipase A2, Calcium Independent) Sandwich ELISA Kit Ready-To-Use (STJE0016426)
产品资料
Human iPLA2 (Phospholipase A2, Calcium Independent) Sandwich ELISA Kit Ready-To-Use (STJE0016426)
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产品名称:
Human iPLA2 (Phospholipase A2, Calcium Independent) Sandwich ELISA Kit Ready-To-Use (STJE0016426)
产品型号:
STJE0016426
产品展商:
ST john's laboratory
产品文档:
无相关文档
简单介绍
Human iPLA2 (Phospholipase A2, Calcium Independent) Sandwich ELISA Kit Ready-To-Use (STJE0016426)
产品描述
Applications:
ELISA
Reactivity:
Human
Note:
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity:
32pg/mL
Detection Limit:
78.1-5000pg/mL
Short Description:
This iPLA2 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction:
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:
3 hrs
Gene Symbol:
PLA2G6
Gene ID:
8398
Uniprot ID:
PLPL9_HUMAN
Immunogen Region:
Ready-To-Use
Sample Type:
serum, plasma or other biological fluids.
Tissue Specificity
Four different transcripts were found to be expressed in a distinct tissue distribution.
Post Translational Modifications
Function
Calcium-independent phospholipase involved in phospholipid remodeling with implications in cellular membrane homeostasis, mitochondrial integrity and signal transduction. Hydrolyzes the ester bond of the fatty acyl group attached at sn-1 or sn-2 position of phospholipids (phospholipase A1 and A2 activity respectively), producing lysophospholipids that are used in deacylation-reacylation cycles. Hydrolyzes both saturated and unsaturated long fatty acyl chains in various glycerophospholipid classes such as phosphatidylcholines, phosphatidylethanolamines and phosphatidates, with a preference for hydrolysis at sn-2 position. Can further hydrolyze lysophospholipids carrying saturated fatty acyl chains (lysophospholipase activity). Upon oxidative stress, contributes to remodeling of mitochondrial phospholipids in pancreatic beta cells, in a repair mechanism to reduce oxidized lipid content. Preferentially hydrolyzes oxidized polyunsaturated fatty acyl chains from cardiolipins, yielding monolysocardiolipins that can be reacylated with unoxidized fatty acyls to regenerate native cardiolipin species. Hydrolyzes oxidized glycerophosphoethanolamines present in pancreatic islets, releasing oxidized polyunsaturated fatty acids such as hydroxyeicosatetraenoates (HETEs). Has thioesterase activity toward fatty-acyl CoA releasing CoA-SH known to facilitate fatty acid transport and beta-oxidation in mitochondria particularly in skeletal muscle. Plays a role in regulation of membrane dynamics and homeostasis. Selectively hydrolyzes sn-2 arachidonoyl group in plasmalogen phospholipids, structural components of lipid rafts and myelin. Regulates F-actin polymerization at the pseudopods, which is required for both speed and directionality of MCP1/CCL2-induced monocyte chemotaxis. Targets membrane phospholipids to produce potent lipid signaling messengers. Generates lysophosphatidate (LPA, 1-acyl-glycerol-3-phosphate), which acts via G-protein receptors in various cell types. Has phospholipase A2 activity toward platelet-activating factor (PAF, 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine), likely playing a role in inactivation of this potent pro-inflammatory signaling lipid. In response to glucose, amplifies calcium influx in pancreatic beta cells to promote INS secretion. Isoform Ankyrin-iPLA2-1: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms. Isoform Ankyrin-iPLA2-2: Lacks the catalytic domain and may act as a negative regulator of the catalytically active isoforms.
Protein Name
85/88 Kda Calcium-Independent Phospholipase A2
Cai-Pla2
2-Lysophosphatidylcholine Acylhydrolase
Group Vi Phospholipase A2
Gvi Pla2
Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta
Ipla2-Beta
Palmitoyl-Coa Hydrolase
Patatin-Like Phospholipase Domain-Containing Protein 9
Pnpla9
Database Links
Reactome: R-HSA-1482788
Reactome: R-HSA-1482798
Reactome: R-HSA-1482839
Reactome: R-HSA-2029485
Reactome: R-HSA-6811436
Cellular Localisation
Cytoplasm
Cell Membrane
Mitochondrion
Cell Projection
Pseudopodium
Recruited To The Membrane-Enriched Pseudopods Upon Mcp1/Ccl2 Stimulation In Monocytes
Alternative ELISA Names
85/88 Kda Calcium-Independent Phospholipase A2 ELISA kit
Cai-Pla2 ELISA kit
2-Lysophosphatidylcholine Acylhydrolase ELISA kit
Group Vi Phospholipase A2 ELISA kit
Gvi Pla2 ELISA kit
Intracellular Membrane-Associated Calcium-Independent Phospholipase A2 Beta ELISA kit
Ipla2-Beta ELISA kit
Palmitoyl-Coa Hydrolase ELISA kit
Patatin-Like Phospholipase Domain-Containing Protein 9 ELISA kit
Pnpla9 ELISA kit
PLA2G6 ELISA kit
PLPLA9 ELISA kit
output
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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