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Human KERA (Keratocan) CLIA Kit (STJC0000788)

Human KERA (Keratocan) CLIA Kit (STJC0000788)
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  • 产品名称:Human KERA (Keratocan) CLIA Kit (STJC0000788)
  • 产品型号:STJC0000788
  • 产品展商:ST john's laboratory
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简单介绍
Human KERA (Keratocan) CLIA Kit (STJC0000788)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 37.5pg/mL
Detection Limit: 62.5~4000pg/mL
Short Description: This human KERA kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: KERA
Gene ID: 11081
Uniprot ID: KERA_HUMAN
Specificity: This kit recognizes Human KERA in samples. No significant cross-reactivity or interference between Human KERA and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Cornea (at protein level). Increased expression in the stroma of keratoconus corneas. Also detected in trachea, and in low levels, in intestine, skeletal muscle, ovary, lung and putamen.
Post Translational Modifications Binds keratan sulfate chains.
Function May be important in developing and maintaining corneal transparency and for the structure of the stromal matrix.
Protein Name Keratocan
Ktn
Keratan Sulfate Proteoglycan Keratocan
Database Links Reactome: R-HSA-2022854
Reactome: R-HSA-2022857
Reactome: R-HSA-3656225
Reactome: R-HSA-3656243
Reactome: R-HSA-3656244
Cellular Localisation Secreted
Extracellular Space
Extracellular Matrix
Alternative CLIA Names Keratocan CLIA kit
Ktn CLIA kit
Keratan Sulfate Proteoglycan Keratocan CLIA kit
KERA CLIA kit
SLRR2B CLIA kit
Specificity This kit recognizes Human KERA in samples. No significant cross-reactivity or interference between Human KERA and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 94
EDTA plasma(n=8) 93-107 101
Cell culture media(n=8) 86-101 92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 207.55 479.68 1989.82 190.72 473.16 2096.27
Standard deviation 26.77 46.82 128.54 24.51 53.94 148.21
CV (%) 12.90 9.76 6.46 12.85 11.40 7.07
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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