Human KITLG (Kit ligand) CLIA Kit (STJC0000601) STJC0000601 上海玉博生物科技有限公司

Human KITLG (Kit ligand) CLIA Kit (STJC0000601)

如果您对该产品感兴趣的话，可以
产品名称：Human KITLG (Kit ligand) CLIA Kit (STJC0000601)
产品型号：STJC0000601
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human KITLG (Kit ligand) CLIA Kit (STJC0000601)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	9.38pg/mL
Detection Limit:	15.63~1000pg/mL
Short Description:	This human KITLG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	KITLG
Gene ID:	4254
Uniprot ID:	SCF_HUMAN
Specificity:	This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain. Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97. A soluble form exists as a cleavage product of the extracellular domain.
Function	Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Protein Name	Kit Ligand Mast Cell Growth Factor Mgf Stem Cell Factor Scf C-Kit Ligand Cleaved Into - Soluble Kit Ligand Skitlg
Database Links	Reactome: R-HSA-1257604 P21583-1 Reactome: R-HSA-1433557 P21583-1 Reactome: R-HSA-1433559 P21583-1 Reactome: R-HSA-2219530 P21583-1 Reactome: R-HSA-5673001 P21583-1 Reactome: R-HSA-6811558 P21583-1
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type I Membrane Protein Isoform 2: Cytoplasm Cytoplasm Cytoskeleton Cell Membrane Cell Projection Lamellipodium Filopodium Soluble Kit Ligand: Secreted
Alternative CLIA Names	Kit Ligand CLIA kit Mast Cell Growth Factor CLIA kit Mgf CLIA kit Stem Cell Factor CLIA kit Scf CLIA kit C-Kit Ligand Cleaved Into - Soluble Kit Ligand CLIA kit Skitlg CLIA kit KITLG CLIA kit MGF CLIA kit SCF CLIA kit
Specificity	This kit recognizes Human SCF in samples. No significant cross-reactivity or interference between Human SCF and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	91-105	98
EDTA plasma(n=8)	93-105	100
Cell culture media(n=8)	88-102	93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	52.31	133.07	424.59	52.42	140.94	385.49
Standard deviation	5.26	10.22	42.59	5.23	12.15	25.44
CV (%)	10.06	7.68	10.03	9.98	8.62	6.60

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报

产品目录

产品资料

Human KITLG (Kit ligand) CLIA Kit (STJC0000601)