Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA (STJE0003429) STJE0003429 上海玉博生物科技有限公司

Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA (STJE0003429)

简单介绍

Human KRT18 (Keratin, type I cytoskeletal 18) Sandwich ELISA (STJE0003429)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	3.75mIU/mL
Detection Limit:	6.25~400mIU/mL
Short Description:	This KRT18 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	KRT18
Gene ID:	3875
Uniprot ID:	K1C18_HUMAN
Specificity:	This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Function	Involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Protein Name	Keratin - Type I Cytoskeletal 18 Cell Proliferation-Inducing Gene 46 Protein Cytokeratin-18 Ck-18 Keratin-18 K18
Database Links	Reactome: R-HSA-6805567 Reactome: R-HSA-6809371
Cellular Localisation	Nucleus Matrix Cytoplasm Perinuclear Region Nucleus Nucleolus
Alternative ELISA Names	Keratin - Type I Cytoskeletal 18 ELISA kit Cell Proliferation-Inducing Gene 46 Protein ELISA kit Cytokeratin-18 ELISA kit Ck-18 ELISA kit Keratin-18 ELISA kit K18 ELISA kit KRT18 ELISA kit CYK18 ELISA kit PIG46 ELISA kit
Specificity	This kit recognizes Human CK-18/KRT18 in samples. No significant cross-reactivity or interference between Human CK-18/KRT18 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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