Human LGALS1 (Galectin-1) CLIA Kit (STJC0000500) STJC0000500 上海玉博生物科技有限公司

Human LGALS1 (Galectin-1) CLIA Kit (STJC0000500)

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产品名称：Human LGALS1 (Galectin-1) CLIA Kit (STJC0000500)
产品型号：STJC0000500
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human LGALS1 (Galectin-1) CLIA Kit (STJC0000500)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This human LGALS1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	LGALS1
Gene ID:	3956
Uniprot ID:	LEG1_HUMAN
Specificity:	This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion (at protein level). Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
Function	Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.
Protein Name	Galectin-1 Gal-1 14 Kda Laminin-Binding Protein Hlbp14 14 Kda Lectin Beta-Galactoside-Binding Lectin L-14-I Galaptin Hbl Hpl Lactose-Binding Lectin 1 Lectin Galactoside-Binding Soluble 1 Putative Mapk-Activating Protein Pm12 S-Lac Lectin 1
Database Links	Reactome: R-HSA-381426 Reactome: R-HSA-8957275
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Cytoplasm Can Be Secreted The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative CLIA Names	Galectin-1 CLIA kit Gal-1 CLIA kit 14 Kda Laminin-Binding Protein CLIA kit Hlbp14 CLIA kit 14 Kda Lectin CLIA kit Beta-Galactoside-Binding Lectin L-14-I CLIA kit Galaptin CLIA kit Hbl CLIA kit Hpl CLIA kit Lactose-Binding Lectin 1 CLIA kit Lectin Galactoside-Binding Soluble 1 CLIA kit Putative Mapk-Activating Protein Pm12 CLIA kit S-Lac Lectin 1 CLIA kit LGALS1 CLIA kit
Specificity	This kit recognizes Human GAL1 in samples. No significant cross-reactivity or interference between Human GAL1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	87-101	93
EDTA plasma(n=8)	91-103	98
Cell culture media(n=8)	94-107	100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.52	1.44	3.42	0.48	1.43	3.59
Standard deviation	0.06	0.15	0.27	0.06	0.11	0.22
CV (%)	11.54	10.42	7.89	12.50	7.69	6.13

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human LGALS1 (Galectin-1) CLIA Kit (STJC0000500)