Human LGALS3 (Galectin-3) CLIA Kit (STJC0000659) STJC0000659 上海玉博生物科技有限公司

Human LGALS3 (Galectin-3) CLIA Kit (STJC0000659)

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产品名称：Human LGALS3 (Galectin-3) CLIA Kit (STJC0000659)
产品型号：STJC0000659
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human LGALS3 (Galectin-3) CLIA Kit (STJC0000659)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human LGALS3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	LGALS3
Gene ID:	3958
Uniprot ID:	LEG3_HUMAN
Specificity:	This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.
Function	Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis. In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. Together with TRIM16, coordinates the recognition of membrane damage with mobilization of the core autophagy regulators ATG16L1 and BECN1 in response to damaged endomembranes.
Protein Name	Galectin-3 Gal-3 35 Kda Lectin Carbohydrate-Binding Protein 35 Cbp 35 Galactose-Specific Lectin 3 Galactoside-Binding Protein Galbp Ige-Binding Protein L-31 Laminin-Binding Protein Lectin L-29 Mac-2 Antigen
Database Links	Reactome: R-HSA-6798695 Reactome: R-HSA-879415 Reactome: R-HSA-8939246 Reactome: R-HSA-8941333
Cellular Localisation	Cytoplasm Nucleus Secreted Secreted By A Non-Classical Secretory Pathway And Associates With The Cell Surface Can Be Secreted The Secretion Is Dependent On Protein Unfolding And Facilitated By The Cargo Receptor Tmed10 It Results In Protein Translocation From The Cytoplasm Into The Ergic (Endoplasmic Reticulum-Golgi Intermediate Compartment) Followed By Vesicle Entry And Secretion
Alternative CLIA Names	Galectin-3 CLIA kit Gal-3 CLIA kit 35 Kda Lectin CLIA kit Carbohydrate-Binding Protein 35 CLIA kit Cbp 35 CLIA kit Galactose-Specific Lectin 3 CLIA kit Galactoside-Binding Protein CLIA kit Galbp CLIA kit Ige-Binding Protein CLIA kit L-31 CLIA kit Laminin-Binding Protein CLIA kit Lectin L-29 CLIA kit Mac-2 Antigen CLIA kit LGALS3 CLIA kit MAC2 CLIA kit
Specificity	This kit recognizes Human GAL3 in samples. No significant cross-reactivity or interference between Human GAL3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	95-111	101
EDTA plasma(n=8)	87-100	94
Cell culture media(n=8)	93-108	99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	104.64	302.32	798.06	110.52	316.19	728.10
Standard deviation	11.30	25.15	72.46	12.88	30.70	74.41
CV (%)	10.80	8.32	9.08	11.65	9.71	10.22

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human LGALS3 (Galectin-3) CLIA Kit (STJC0000659)