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Human LIF (Leukemia inhibitory factor) Sandwich ELISA (STJE0001984)

Human LIF (Leukemia inhibitory factor) Sandwich ELISA (STJE0001984)
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  • 产品名称:Human LIF (Leukemia inhibitory factor) Sandwich ELISA (STJE0001984)
  • 产品型号:STJE0001984
  • 产品展商:ST john's laboratory
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简单介绍
Human LIF (Leukemia inhibitory factor) Sandwich ELISA (STJE0001984)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This LIF Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: LIF
Gene ID: 3976
Uniprot ID: LIF_HUMAN
Specificity: This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Protein Name Leukemia Inhibitory Factor
Lif
Differentiation-Stimulating Factor
D Factor
Melanoma-Derived Lpl Inhibitor
Mlpli
Emfilermin
Database Links Reactome: R-HSA-6783783
Reactome: R-HSA-6785807
Reactome: R-HSA-6788467
Cellular Localisation Secreted
Alternative ELISA Names Leukemia Inhibitory Factor ELISA kit
Lif ELISA kit
Differentiation-Stimulating Factor ELISA kit
D Factor ELISA kit
Melanoma-Derived Lpl Inhibitor ELISA kit
Mlpli ELISA kit
Emfilermin ELISA kit
LIF ELISA kit
HILDA ELISA kit
Specificity This kit recognizes Human LIF in samples. No significant cross-reactivity or interference between Human LIF and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-100 93
EDTA plasma (n=8) 91-102 96
Cell culture media (n=8) 92-104 97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 101.61 298.37 716.65 97.10 285.07 782.41
Standard deviation 6.03 12.14 26.01 5.88 15.17 29.73
CV (%) 5.93 4.07 3.63 6.06 5.32 3.80
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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