Human LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010365) STJE0010365 上海玉博生物科技有限公司

Human LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010365)

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产品名称：Human LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010365)
产品型号：STJE0010365
产品展商：ST john's laboratory
产品文档：无相关文档

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Human LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010365)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity:	0.054ng/mL
Detection Limit:	0.156-10ng/mL
Short Description:	This LMNA Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	4.5 hrs

Gene Symbol:	LMNA
Gene ID:	4000
Uniprot ID:	LMNA_HUMAN
Sample Type:	tissue homogenates or other biological fluids.

Tissue Specificity	In the arteries, prelamin-A/C accumulation is not observed in young healthy vessels but is prevalent in medial vascular smooth muscle cells (VSMCs) from aged individuals and in atherosclerotic lesions, where it often colocalizes with senescent and degenerate VSMCs. Prelamin-A/C expression increases with age and disease. In normal aging, the accumulation of prelamin-A/C is caused in part by the down-regulation of ZMPSTE24/FACE1 in response to oxidative stress.
Post Translational Modifications	Proteolytic cleavage of the C-terminal of 18 residues of prelamin-A/C results in the production of lamin-A/C. The prelamin-A/C maturation pathway includes farnesylation of CAAX motif by protein farnesyltransferase (FNTA and FNTB), removal of the last three amino acids (-AAX) by RCE1/FACE2 and/or ZMPSTE24, methylation of the C-terminal cysteine by ICMT and endoproteolytic removal of the last 15 C-terminal amino acids by ZMPSTE24. Proteolytic cleavage requires prior farnesylation and methylation, and absence of these blocks cleavage. Farnesylation of prelamin-A/C facilitates nuclear envelope targeting. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. Phosphorylation status of S-22 determines its localization between double-strand break (DSB) sites and the nuclear matrix. Sumoylation is necessary for the localization to the nuclear envelope.
Function	Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. Lamin A and C are present in equal amounts in the lamina of mammals. Recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends. Plays an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Required for cardiac homeostasis. Prelamin-A/C can accelerate smooth muscle cell senescence. It acts to disrupt mitosis and induce DNA damage in vascular smooth muscle cells (VSMCs), leading to mitotic failure, genomic instability, and premature senescence.
Protein Name	Prelamin-A/C Cleaved Into - Lamin-A/C 70 Kda Lamin Renal Carcinoma Antigen Ny-Ren-32
Database Links	Reactome: R-HSA-1221632 P02545-2 Reactome: R-HSA-2980766 Reactome: R-HSA-2995383 Reactome: R-HSA-352238 P02545-1 Reactome: R-HSA-381038 Reactome: R-HSA-4419969 Reactome: R-HSA-6802952 Reactome: R-HSA-8862803 P02545-1
Cellular Localisation	Nucleus Nucleus Envelope Nucleus Lamina Nucleoplasm Nucleus Matrix Farnesylation Of Prelamin-A/C Facilitates Nuclear Envelope Targeting And Subsequent Cleavage By Zmpste24/Face1 To Remove The Farnesyl Group Produces Mature Lamin-A/C Which Can Then Be Inserted Into The Nuclear Lamina Emd Is Required For Proper Localization Of Non-Farnesylated Prelamin-A/C Isoform C: Nucleus Speckle
Alternative ELISA Names	Prelamin-A/C Cleaved Into - Lamin-A/C ELISA kit 70 Kda Lamin ELISA kit Renal Carcinoma Antigen Ny-Ren-32 ELISA kit LMNA ELISA kit LMN1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human LMNA (Lamin A/C) Sandwich ELISA Kit (STJE0010365)