Human MBP (Myelin Basic Protein) Sandwich ELISA (STJE0019813) STJE0019813 上海玉博生物科技有限公司

Human MBP (Myelin Basic Protein) Sandwich ELISA (STJE0019813)

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产品名称：Human MBP (Myelin Basic Protein) Sandwich ELISA (STJE0019813)
产品型号：STJE0019813
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MBP (Myelin Basic Protein) Sandwich ELISA (STJE0019813)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This MBP Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	MBP
Gene ID:	4155
Uniprot ID:	MBP_HUMAN
Specificity:	This kit recognizes Human MBP in samples. No significant crossreactivity or interference between Human MBP and analogues was observed.
Sample Type:	serum, plasma and other biological fluids

Tissue Specificity	MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.
Post Translational Modifications	Several charge isomers of MBP.C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form).are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases during aging, making the protein more cationic. The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated. Proteolytically cleaved in B cell lysosomes by cathepsin CTSG which degrades the major immunogenic MBP epitope and prevents the activation of MBP-specific autoreactive T cells. Phosphorylated by TAOK2, VRK2, MAPK11, MAPK12, MAPK14 and MINK1.
Function	The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined with optional post-translational modifications give a wide spectrum of isomers, with each of them potentially having a specialized function. Induces T-cell proliferation.
Protein Name	Myelin Basic Protein Mbp Myelin A1 Protein Myelin Membrane Encephalitogenic Protein
Database Links	Reactome: R-HSA-9619665
Cellular Localisation	Myelin Membrane Peripheral Membrane Protein Cytoplasmic Side Cytoplasmic Side Of Myelin Isoform 3: Nucleus Targeted To Nucleus In Oligodendrocytes
Alternative ELISA Names	Myelin Basic Protein ELISA kit Mbp ELISA kit Myelin A1 Protein ELISA kit Myelin Membrane Encephalitogenic Protein ELISA kit MBP ELISA kit
Specificity	This kit recognizes Human MBP in samples. No significant cross-reactivity or interference between Human MBP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	90-103	95
EDTA plasma (n=8)	91-107	98
Cell culture media (n=8)	88-101	94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	107.04	188.54	921.90	107.47	202.06	900.84
Standard deviation	5.99	10.48	32.54	7.19	9.88	37.93
CV (%)	5.60	5.56	3.53	6.69	4.89	4.21

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MBP (Myelin Basic Protein) Sandwich ELISA (STJE0019813)