Human MECP2 (Methyl-CpG-binding protein 2) Sandwich ELISA (STJE0003828) STJE0003828 上海玉博生物科技有限公司

Human MECP2 (Methyl-CpG-binding protein 2) Sandwich ELISA (STJE0003828)

简单介绍

Human MECP2 (Methyl-CpG-binding protein 2) Sandwich ELISA (STJE0003828)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This MECP2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	MECP2
Gene ID:	4204
Uniprot ID:	MECP2_HUMAN
Specificity:	This kit recognizes Human MeCP2 in samples. No significant cross-reactivity or interference between Human MeCP2 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated on Ser-423 in brain upon synaptic activity, which attenuates its repressor activity and seems to regulate dendritic growth and spine maturation.
Function	Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Protein Name	Methyl-Cpg-Binding Protein 2 Mecp-2 Protein Mecp2
Database Links	Reactome: R-HSA-8986944 Reactome: R-HSA-9022534 Reactome: R-HSA-9022535 Reactome: R-HSA-9022537 Reactome: R-HSA-9022538 Reactome: R-HSA-9022692 Reactome: R-HSA-9022699 Reactome: R-HSA-9022702 Reactome: R-HSA-9022707 Reactome: R-HSA-9022927 Reactome: R-HSA-9725371
Cellular Localisation	Nucleus Colocalized With Methyl-Cpg In The Genome Colocalized With Tbl1x To The Heterochromatin Foci
Alternative ELISA Names	Methyl-Cpg-Binding Protein 2 ELISA kit Mecp-2 Protein ELISA kit Mecp2 ELISA kit MECP2 ELISA kit
Specificity	This kit recognizes Human MeCP2 in samples. No significant cross-reactivity or interference between Human MeCP2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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