Human MIP-1 Beta (Macrophage Inflammatory Protein 1 Beta) Sandwich ELISA (STJE0019862) STJE0019862 上海玉博生物科技有限公司

Human MIP-1 Beta (Macrophage Inflammatory Protein 1 Beta) Sandwich ELISA (STJE0019862)

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产品名称：Human MIP-1 Beta (Macrophage Inflammatory Protein 1 Beta) Sandwich ELISA (STJE0019862)
产品型号：STJE0019862
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MIP-1 Beta (Macrophage Inflammatory Protein 1 Beta) Sandwich ELISA (STJE0019862)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	4.04 pg/mL
Detection Limit:	7.81-500 pg/mL
Short Description:	This MIP-1 Beta Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	CCL4
Gene ID:	388372/6351
Uniprot ID:	CCL4_HUMAN
Specificity:	This kit recognizes Human MIP-1 Beta in samples. No significant cross-reactivity or interference between Human MIP-1 Beta and analogues was observed.
Sample Type:	serum, plasma and other biological fluids

Post Translational Modifications	N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Function	Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Protein Name	C-C Motif Chemokine 4 G-26 T-Lymphocyte-Secreted Protein Hc21 Lymphocyte Activation Gene 1 Protein Lag-1 Mip-1-Beta(1-69 Macrophage Inflammatory Protein 1-Beta Mip-1-Beta Pat 744 Protein H400 Sis-Gamma Small-Inducible Cytokine A4 T-Cell Activation Protein 2 Act-2 Cleaved Into - Mip-1-Beta(3-69
Database Links	Reactome: R-HSA-380108 Reactome: R-HSA-418594 Reactome: R-HSA-6783783
Cellular Localisation	Secreted
Alternative ELISA Names	C-C Motif Chemokine 4 ELISA kit G-26 T-Lymphocyte-Secreted Protein ELISA kit Hc21 ELISA kit Lymphocyte Activation Gene 1 Protein ELISA kit Lag-1 ELISA kit Mip-1-Beta(1-69 ELISA kit Macrophage Inflammatory Protein 1-Beta ELISA kit Mip-1-Beta ELISA kit Pat 744 ELISA kit Protein H400 ELISA kit Sis-Gamma ELISA kit Small-Inducible Cytokine A4 ELISA kit T-Cell Activation Protein 2 ELISA kit Act-2 Cleaved Into - Mip-1-Beta(3-69 ELISA kit CCL4 ELISA kit LAG1 ELISA kit MIP1B ELISA kit SCYA4 ELISA kit
Specificity	This kit recognizes Human MIP-1β in samples. No significant cross-reactivity or interference between Human MIP-1β and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	87-102	94
EDTA plasma (n=8)	90-105	97
Cell culture media (n=8)	90-100	95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	22.84	59.65	199.49	20.60	56.38	190.25
Standard deviation	1.59	3.29	6.80	1.12	2.31	9.36
CV (%)	6.94	5.51	3.41	5.44	4.10	4.92

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MIP-1 Beta (Macrophage Inflammatory Protein 1 Beta) Sandwich ELISA (STJE0019862)