Human MKI67 (Proliferation marker protein Ki-67) Sandwich ELISA (STJE0003837) STJE0003837 上海玉博生物科技有限公司

Human MKI67 (Proliferation marker protein Ki-67) Sandwich ELISA (STJE0003837)

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产品名称：Human MKI67 (Proliferation marker protein Ki-67) Sandwich ELISA (STJE0003837)
产品型号：STJE0003837
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MKI67 (Proliferation marker protein Ki-67) Sandwich ELISA (STJE0003837)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.19ng/mL
Detection Limit:	0.31~20ng/mL
Short Description:	This MKI67 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	MKI67
Gene ID:	4288
Uniprot ID:	KI67_HUMAN
Specificity:	This kit recognizes Human Ki67P in samples. No significant cross-reactivity or interference between Human Ki67P and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly. Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface. Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility. Binds DNA, with a preference for supercoiled DNA and AT-rich DNA. Does not contribute to the internal structure of mitotic chromosomes. May play a role in chromatin organization. It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Protein Name	Proliferation Marker Protein Ki-67 Antigen Identified By Monoclonal Antibody Ki-67 Antigen Ki-67 Antigen Ki67
Cellular Localisation	Chromosome Nucleus Nucleolus Associates With The Surface Of The Mitotic Chromosome The Perichromosomal Layer And Covers A Substantial Fraction Of The Mitotic Chromosome Surface Associates With Satellite Dna In G1 Phase Binds Tightly To Chromatin In Interphase Chromatin-Binding Decreases In Mitosis When It Associates With The Surface Of The Condensed Chromosomes Predominantly Localized In The G1 Phase In The Perinucleolar Region In The Later Phases It Is Also Detected Throughout The Nuclear Interior Being Predominantly Localized In The Nuclear Matrix
Alternative ELISA Names	Proliferation Marker Protein Ki-67 ELISA kit Antigen Identified By Monoclonal Antibody Ki-67 ELISA kit Antigen Ki-67 ELISA kit Antigen Ki67 ELISA kit MKI67 ELISA kit
Specificity	This kit recognizes Human Ki67P in samples. No significant cross-reactivity or interference between Human Ki67P and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	90-105	96
EDTA plasma (n=8)	95-109	102
Cell culture media (n=8)	91-107	99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.92	3.10	9.40	0.83	3.26	8.87
Standard deviation	0.05	0.13	0.49	0.04	0.17	0.38
CV (%)	5.43	4.19	5.21	4.82	5.21	4.28

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MKI67 (Proliferation marker protein Ki-67) Sandwich ELISA (STJE0003837)