Human MME (Neprilysin) Sandwich ELISA (STJE0002492) STJE0002492 上海玉博生物科技有限公司

Human MME (Neprilysin) Sandwich ELISA (STJE0002492)

简单介绍

Human MME (Neprilysin) Sandwich ELISA (STJE0002492)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This MME Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	MME
Gene ID:	4311
Uniprot ID:	NEP_HUMAN
Specificity:	This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Catalyzes cleavage of bradykinin, substance P and neurotensin peptides. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) and brain natriuretic factor (BNP(1-32)). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Protein Name	Neprilysin Atriopeptidase Common Acute Lymphocytic Leukemia Antigen Calla Enkephalinase Neutral Endopeptidase 24.11 Nep Neutral Endopeptidase Skin Fibroblast Elastase Sfe Cd Antigen Cd10
Database Links	Reactome: R-HSA-2022377 Reactome: R-HSA-5578768 Reactome: R-HSA-6798695
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein
Alternative ELISA Names	Neprilysin ELISA kit Atriopeptidase ELISA kit Common Acute Lymphocytic Leukemia Antigen ELISA kit Calla ELISA kit Enkephalinase ELISA kit Neutral Endopeptidase 24.11 ELISA kit Nep ELISA kit Neutral Endopeptidase ELISA kit Skin Fibroblast Elastase ELISA kit Sfe ELISA kit Cd Antigen Cd10 ELISA kit MME ELISA kit EPN ELISA kit
Specificity	This kit recognizes Human NEP in samples. No significant cross-reactivity or interference between Human NEP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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