Human MMP13 (Collagenase 3) CLIA Kit (STJC0000114) STJC0000114 上海玉博生物科技有限公司

Human MMP13 (Collagenase 3) CLIA Kit (STJC0000114)

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产品名称：Human MMP13 (Collagenase 3) CLIA Kit (STJC0000114)
产品型号：STJC0000114
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MMP13 (Collagenase 3) CLIA Kit (STJC0000114)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	46.88pg/mL
Detection Limit:	78.13~5000pg/mL
Short Description:	This human MMP13 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	MMP13
Gene ID:	4322
Uniprot ID:	MMP13_HUMAN
Specificity:	This kit recognizes Human MMP-13 in samples. No significant cross-reactivity or interference between Human MMP-13 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Detected in chondrocytes from in joint cartilage that have been treated with TNF and IL1B, but not in untreated chondrocytes. Detected in T lymphocytes. Detected in breast carcinoma tissue.
Post Translational Modifications	The proenzyme is activated by removal of the propeptide.this cleavage can be effected by other matrix metalloproteinases, such as MMP2, MMP3 and MMP14 and may involve several cleavage steps. Cleavage can also be autocatalytic, after partial maturation by another protease or after treatment with 4-aminophenylmercuric acetate (APMA) (in vitro). N-glycosylated. Tyrosine phosphorylated by PKDCC/VLK.
Function	Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.
Protein Name	Collagenase 3 Matrix Metalloproteinase-13 Mmp-13
Database Links	Reactome: R-HSA-1442490 Reactome: R-HSA-1474228 Reactome: R-HSA-1592389 Reactome: R-HSA-2022090 Reactome: R-HSA-8941332
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix
Alternative CLIA Names	Collagenase 3 CLIA kit Matrix Metalloproteinase-13 CLIA kit Mmp-13 CLIA kit MMP13 CLIA kit
Specificity	This kit recognizes Human MMP-13 in samples. No significant cross-reactivity or interference between Human MMP-13 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	99-116	106
EDTA plasma(n=8)	89-104	95
Cell culture media(n=8)	99-115	106

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	248.29	763.90	2041.85	249.21	747.59	1876.96
Standard deviation	23.31	62.41	176.21	22.85	79.32	126.51
CV (%)	9.39	8.17	8.63	9.17	10.61	6.74

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MMP13 (Collagenase 3) CLIA Kit (STJC0000114)