Human MUSK (Muscle, skeletal receptor tyrosine-protein kinase) Sandwich ELISA (STJE0002897) STJE0002897 上海玉博生物科技有限公司

Human MUSK (Muscle, skeletal receptor tyrosine-protein kinase) Sandwich ELISA (STJE0002897)

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产品名称：Human MUSK (Muscle, skeletal receptor tyrosine-protein kinase) Sandwich ELISA (STJE0002897)
产品型号：STJE0002897
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MUSK (Muscle, skeletal receptor tyrosine-protein kinase) Sandwich ELISA (STJE0002897)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This MUSK Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	MUSK
Gene ID:	4593
Uniprot ID:	MUSK_HUMAN
Specificity:	This kit recognizes Human MUSK in samples. No significant cross-reactivity or interference between Human MUSK and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation.
Protein Name	Muscle - Skeletal Receptor Tyrosine-Protein Kinase Muscle-Specific Tyrosine-Protein Kinase Receptor Musk Muscle-Specific Kinase Receptor
Database Links	Reactome: R-HSA-3000178
Cellular Localisation	Postsynaptic Cell Membrane Single-Pass Type I Membrane Protein Colocalizes With Acetylcholine Receptors (Achr) To The Postsynaptic Cell Membrane Of The Neuromuscular Junction
Alternative ELISA Names	Muscle - Skeletal Receptor Tyrosine-Protein Kinase ELISA kit Muscle-Specific Tyrosine-Protein Kinase Receptor ELISA kit Musk ELISA kit Muscle-Specific Kinase Receptor ELISA kit MUSK ELISA kit
Specificity	This kit recognizes Human MUSK in samples. No significant cross-reactivity or interference between Human MUSK and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-107	100
EDTA plasma (n=8)	84-98	91
Cell culture media (n=8)	94-107	101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.55	1.38	3.97	0.55	1.38	3.96
Standard deviation	0.04	0.07	0.20	0.04	0.07	0.18
CV (%)	7.27	5.07	5.04	7.27	5.07	4.55

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MUSK (Muscle, skeletal receptor tyrosine-protein kinase) Sandwich ELISA (STJE0002897)