Human MYH10 (Myosin-10) CLIA Kit (STJC0000375) STJC0000375 上海玉博生物科技有限公司

Human MYH10 (Myosin-10) CLIA Kit (STJC0000375)

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产品名称：Human MYH10 (Myosin-10) CLIA Kit (STJC0000375)
产品型号：STJC0000375
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MYH10 (Myosin-10) CLIA Kit (STJC0000375)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human MYH10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	MYH10
Gene ID:	4628
Uniprot ID:	MYH10_HUMAN
Specificity:	This kit recognizes Human MYH10 in samples. No significant cross-reactivity or interference between Human MYH10 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
Post Translational Modifications	Phosphorylated by ABL2.
Function	Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. During cell spreading, plays an important role in cytoskeleton reorganization, focal contacts formation (in the central part but not the margins of spreading cells), and lamellipodial extension.this function is mechanically antagonized by MYH9.
Protein Name	Myosin-10 Cellular Myosin Heavy Chain - Type B Myosin Heavy Chain 10 Myosin Heavy Chain - Non-Muscle Iib Non-Muscle Myosin Heavy Chain B Nmmhc-B Non-Muscle Myosin Heavy Chain Iib Nmmhc Ii-B Nmmhc-Iib
Database Links	Reactome: R-HSA-3928663 Reactome: R-HSA-416572 Reactome: R-HSA-5625740 Reactome: R-HSA-5625900 Reactome: R-HSA-5627117 Reactome: R-HSA-5627123
Cellular Localisation	Cell Projection Lamellipodium Colocalizes With Mcc At The Leading Edge Of Migrating Cells
Alternative CLIA Names	Myosin-10 CLIA kit Cellular Myosin Heavy Chain - Type B CLIA kit Myosin Heavy Chain 10 CLIA kit Myosin Heavy Chain - Non-Muscle Iib CLIA kit Non-Muscle Myosin Heavy Chain B CLIA kit Nmmhc-B CLIA kit Non-Muscle Myosin Heavy Chain Iib CLIA kit Nmmhc Ii-B CLIA kit Nmmhc-Iib CLIA kit MYH10 CLIA kit
Specificity	This kit recognizes Human MYH10 in samples. No significant cross-reactivity or interference between Human MYH10 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	85-100	91
EDTA plasma(n=8)	96-111	103
Cell culture media(n=8)	94-109	100

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	94.83	216.35	805.12	93.87	224.66	791.66
Standard deviation	9.67	17.09	85.58	9.57	19.68	52.49
CV (%)	10.20	7.90	10.63	10.19	8.76	6.63

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MYH10 (Myosin-10) CLIA Kit (STJC0000375)