Human MYH9 (Myosin-9) CLIA Kit (STJC0000376) STJC0000376 上海玉博生物科技有限公司

Human MYH9 (Myosin-9) CLIA Kit (STJC0000376)

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产品名称：Human MYH9 (Myosin-9) CLIA Kit (STJC0000376)
产品型号：STJC0000376
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human MYH9 (Myosin-9) CLIA Kit (STJC0000376)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This human MYH9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	MYH9
Gene ID:	4627
Uniprot ID:	MYH9_HUMAN
Specificity:	This kit recognizes Human MYH9 in samples. No significant cross-reactivity or interference between Human MYH9 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	In the kidney, expressed in the glomeruli. Also expressed in leukocytes.
Post Translational Modifications	ISGylated. Ubiquitination.
Function	Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Required for cortical actin clearance prior to oocyte exocytosis. Promotes cell motility in conjunction with S100A4. During cell spreading, plays an important role in cytoskeleton reorganization, focal contact formation (in the margins but not the central part of spreading cells), and lamellipodial retraction.this function is mechanically antagonized by MYH10.
Protein Name	Myosin-9 Cellular Myosin Heavy Chain - Type A Myosin Heavy Chain 9 Myosin Heavy Chain - Non-Muscle Iia Non-Muscle Myosin Heavy Chain A Nmmhc-A Non-Muscle Myosin Heavy Chain Iia Nmmhc Ii-A Nmmhc-Iia
Database Links	Reactome: R-HSA-1445148 Reactome: R-HSA-2029482 Reactome: R-HSA-3928663 Reactome: R-HSA-416572 Reactome: R-HSA-5625740 Reactome: R-HSA-5625900 Reactome: R-HSA-5627117 Reactome: R-HSA-5627123 Reactome: R-HSA-9662360 Reactome: R-HSA-9662361 Reactome: R-HSA-9662834 Reactome: R-HSA-9664422 Reactome: R-HSA-9725370
Cellular Localisation	Cytoplasm Cytoskeleton Cell Cortex Cytoplasmic Vesicle Secretory Vesicle Cortical Granule Colocalizes With Actin Filaments At Lamellipodia Margins And At The Leading Edge Of Migrating Cells In Retinal Pigment Epithelial Cells Predominantly Localized To Stress Fiber-Like Structures With Some Localization To Cytoplasmic Puncta
Alternative CLIA Names	Myosin-9 CLIA kit Cellular Myosin Heavy Chain - Type A CLIA kit Myosin Heavy Chain 9 CLIA kit Myosin Heavy Chain - Non-Muscle Iia CLIA kit Non-Muscle Myosin Heavy Chain A CLIA kit Nmmhc-A CLIA kit Non-Muscle Myosin Heavy Chain Iia CLIA kit Nmmhc Ii-A CLIA kit Nmmhc-Iia CLIA kit MYH9 CLIA kit
Specificity	This kit recognizes Human MYH9 in samples. No significant cross-reactivity or interference between Human MYH9 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	97-111	104
EDTA plasma(n=8)	99-114	107
Cell culture media(n=8)	85-95	90

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	102.11	253.63	828.04	92.51	258.30	785.16
Standard deviation	12.89	28.51	84.29	7.65	25.98	82.99
CV (%)	12.62	11.24	10.18	8.27	10.06	10.57

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human MYH9 (Myosin-9) CLIA Kit (STJC0000376)