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Human MYL9 (Myosin regulatory light polypeptide 9) CLIA Kit (STJC0000377)

Human MYL9 (Myosin regulatory light polypeptide 9) CLIA Kit (STJC0000377)
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  • 产品名称:Human MYL9 (Myosin regulatory light polypeptide 9) CLIA Kit (STJC0000377)
  • 产品型号:STJC0000377
  • 产品展商:ST john's laboratory
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简单介绍
Human MYL9 (Myosin regulatory light polypeptide 9) CLIA Kit (STJC0000377)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human MYL9 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: MYL9
Gene ID: 10398
Uniprot ID: MYL9_HUMAN
Specificity: This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Smooth muscle tissues and in some, but not all, nonmuscle cells.
Post Translational Modifications Phosphorylation increases the actin-activated myosin ATPase activity and thereby regulates the contractile activity. It is required to generate the driving force in the migration of the cells but not necessary for localization of myosin-2 at the leading edge. Phosphorylation is required for myotube formation.
Function Myosin regulatory subunit that plays an important role in regulation of both smooth muscle and nonmuscle cell contractile activity via its phosphorylation. Implicated in cytokinesis, receptor capping, and cell locomotion. In myoblasts, may regulate PIEZO1-dependent cortical actomyosin assembly involved in myotube formation.
Protein Name Myosin Regulatory Light Polypeptide 9
20 Kda Myosin Light Chain
Lc20
Mlc-2c
Myosin Rlc
Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform
Myosin Regulatory Light Chain 9
Myosin Regulatory Light Chain Mrlc1
Database Links Reactome: R-HSA-3928663
Reactome: R-HSA-416572
Reactome: R-HSA-445355
Reactome: R-HSA-5625740
Reactome: R-HSA-5625900
Reactome: R-HSA-5627117
Reactome: R-HSA-5627123
Reactome: R-HSA-8936459
Cellular Localisation Cytoplasm
Cytoskeleton
Cell Cortex
Colocalizes With F-Actin
Myh9 And Piezo1 At The Actomyosin Cortex In Myoblasts
Alternative CLIA Names Myosin Regulatory Light Polypeptide 9 CLIA kit
20 Kda Myosin Light Chain CLIA kit
Lc20 CLIA kit
Mlc-2c CLIA kit
Myosin Rlc CLIA kit
Myosin Regulatory Light Chain 2 - Smooth Muscle Isoform CLIA kit
Myosin Regulatory Light Chain 9 CLIA kit
Myosin Regulatory Light Chain Mrlc1 CLIA kit
MYL9 CLIA kit
MLC2 CLIA kit
MRLC1 CLIA kit
MYRL2 CLIA kit
Specificity This kit recognizes Human MYL9 in samples. No significant cross-reactivity or interference between Human MYL9 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 100
EDTA plasma(n=8) 89-102 94
Cell culture media(n=8) 97-112 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 51.79 84.09 372.03 48.62 89.88 400.18
Standard deviation 5.96 6.25 27.12 5.84 8.39 38.10
CV (%) 11.51 7.43 7.29 12.01 9.33 9.52
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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