Human NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA (STJE0019870) STJE0019870 上海玉博生物科技有限公司

Human NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA (STJE0019870)

简单介绍

Human NEFL (Neurofilament, Light Polypeptide) Sandwich ELISA (STJE0019870)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	1.03 pg/mL
Detection Limit:	3.13-200 pg/mL
Short Description:	This NEFL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	NEFL
Gene ID:	4747
Uniprot ID:	NFL_HUMAN
Specificity:	This kit recognizes Human NEFL in samples. No significant cross-reactivity or interference between Human NEFL and analogues was observed.
Sample Type:	serum, plasma and other biological fluids

Post Translational Modifications	O-glycosylated. Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization. Ubiquitinated in the presence of TRIM2 and UBE2D1.
Function	Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
Protein Name	Neurofilament Light Polypeptide Nf-L 68 Kda Neurofilament Protein Neurofilament Triplet L Protein
Database Links	Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-5673001 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244
Cellular Localisation	Cell Projection Axon Cytoplasm Cytoskeleton
Alternative ELISA Names	Neurofilament Light Polypeptide ELISA kit Nf-L ELISA kit 68 Kda Neurofilament Protein ELISA kit Neurofilament Triplet L Protein ELISA kit NEFL ELISA kit NF68 ELISA kit NFL ELISA kit
Specificity	This kit recognizes Human NEFL in samples. No significant cross-reactivity or interference between Human NEFL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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