Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA (STJE0002447) STJE0002447 上海玉博生物科技有限公司

Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA (STJE0002447)

简单介绍

Human NOS1 (Nitric oxide synthase, brain) Sandwich ELISA (STJE0002447)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.09ng/mL
Detection Limit:	0.16~10ng/mL
Short Description:	This NOS1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	NOS1
Gene ID:	4842
Uniprot ID:	NOS1_HUMAN
Specificity:	This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Ubiquitinated.mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
Protein Name	Nitric Oxide Synthase 1 Constitutive Nos Nc-Nos Nos Type I Neuronal Nos N-Nos Nnos Nitric Oxide Synthase - Brain Bnos Peptidyl-Cysteine S-Nitrosylase Nos1
Database Links	Reactome: R-HSA-1222556 Reactome: R-HSA-392154 Reactome: R-HSA-5578775
Cellular Localisation	Cell Membrane Sarcolemma Peripheral Membrane Protein Cell Projection Dendritic Spine In Skeletal Muscle It Is Localized Beneath The Sarcolemma Of Fast-Twitch Muscle Fiber By Associating With The Dystrophin Glycoprotein Complex In Neurons Enriched In Dendritic Spines
Alternative ELISA Names	Nitric Oxide Synthase 1 ELISA kit Constitutive Nos ELISA kit Nc-Nos ELISA kit Nos Type I ELISA kit Neuronal Nos ELISA kit N-Nos ELISA kit Nnos ELISA kit Nitric Oxide Synthase - Brain ELISA kit Bnos ELISA kit Peptidyl-Cysteine S-Nitrosylase Nos1 ELISA kit NOS1 ELISA kit
Specificity	This kit recognizes Human NOS1/nNOS in samples. No significant cross-reactivity or interference between Human NOS1/nNOS and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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