Human NOX5 (NADPH oxidase 5) Sandwich ELISA (STJE0003542) STJE0003542 上海玉博生物科技有限公司

Human NOX5 (NADPH oxidase 5) Sandwich ELISA (STJE0003542)

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产品名称：Human NOX5 (NADPH oxidase 5) Sandwich ELISA (STJE0003542)
产品型号：STJE0003542
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human NOX5 (NADPH oxidase 5) Sandwich ELISA (STJE0003542)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This NOX5 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	NOX5
Gene ID:	79400
Uniprot ID:	NOX5_HUMAN
Specificity:	This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Isoform v2: Phosphorylation at Ser-475 by CaMK2 and at Ser-490, Thr-494 and Ser-498 by PKC/PRKCA positively regulates its catalytic activity. Isoform v2: S-nitrosylation in response to nitric oxide inhibits its catalytic activity.
Function	Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. May play a role in cell growth and apoptosis. Isoform v2: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Also functions as a calcium-dependent proton channel and may regulate redox-dependent processes in lymphocytes and spermatozoa. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v1: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. Isoform v5: Calcium-dependent NADPH oxidase that catalyzes the generation of superoxide from molecular oxygen utilizing NADPH as an electron donor. lacks enzyme activity. Involved in endothelial generation of reactive oxygen species (ROS), proliferation and angiogenesis and contribute to endothelial response to thrombin. Isoform v4: Lacks calcium-dependent NADPH oxidase activity. Isoform v3: Lacks calcium-dependent NADPH oxidase activity.
Protein Name	Nadph Oxidase 5
Database Links	Reactome: R-HSA-3299685
Cellular Localisation	Isoform V2: Endoplasmic Reticulum Cell Membrane Multi-Pass Membrane Protein Calcium-Sensitive Association And Dissociation Between The N- And C-Terminal Domains Appears To Facilitate Its Localization To The Cell Membrane Isoform V5: Endoplasmic Reticulum
Alternative ELISA Names	Nadph Oxidase 5 ELISA kit NOX5 ELISA kit
Specificity	This kit recognizes Human NOX5 in samples. No significant cross-reactivity or interference between Human NOX5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-107	100
EDTA plasma (n=8)	88-101	93
Cell culture media (n=8)	87-99	93

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	95.89	220.44	938.13	97.64	229.99	959.29
Standard deviation	4.95	8.88	48.13	6.17	12.90	39.14
CV (%)	5.16	4.03	5.13	6.32	5.61	4.08

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human NOX5 (NADPH oxidase 5) Sandwich ELISA (STJE0003542)