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Human NR1I2 (Nuclear receptor subfamily 1 group I member 2) CLIA Kit (STJC0000985)

Human NR1I2 (Nuclear receptor subfamily 1 group I member 2) CLIA Kit (STJC0000985)
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  • 产品名称:Human NR1I2 (Nuclear receptor subfamily 1 group I member 2) CLIA Kit (STJC0000985)
  • 产品型号:STJC0000985
  • 产品展商:ST john's laboratory
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简单介绍
Human NR1I2 (Nuclear receptor subfamily 1 group I member 2) CLIA Kit (STJC0000985)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This human NR1I2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: NR1I2
Gene ID: 8856
Uniprot ID: NR1I2_HUMAN
Specificity: This kit recognizes Human PXR in samples. No significant cross-reactivity or interference between Human PXR and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Expressed in liver, colon and small intestine.
Function Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds. Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone. Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes.
Protein Name Nuclear Receptor Subfamily 1 Group I Member 2
Orphan Nuclear Receptor Par1
Orphan Nuclear Receptor Pxr
Pregnane X Receptor
Steroid And Xenobiotic Receptor
Sxr
Database Links Reactome: R-HSA-383280
Reactome: R-HSA-4090294 O75469-1
Cellular Localisation Nucleus
Alternative CLIA Names Nuclear Receptor Subfamily 1 Group I Member 2 CLIA kit
Orphan Nuclear Receptor Par1 CLIA kit
Orphan Nuclear Receptor Pxr CLIA kit
Pregnane X Receptor CLIA kit
Steroid And Xenobiotic Receptor CLIA kit
Sxr CLIA kit
NR1I2 CLIA kit
PXR CLIA kit
Specificity This kit recognizes Human PXR in samples. No significant cross-reactivity or interference between Human PXR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 90-106 98
EDTA plasma(n=8) 95-111 101
Cell culture media(n=8) 92-104 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 104.20 206.55 908.49 111.55 203.46 981.23
Standard deviation 9.47 22.22 63.59 11.67 14.38 110.29
CV (%) 9.09 10.76 7.00 10.46 7.07 11.24
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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