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Human NRG1 (Neuregulin 1) Sandwich ELISA (STJE0004104)
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Human NRG1 (Neuregulin 1) Sandwich ELISA (STJE0004104)
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Human NRG1 (Neuregulin 1) Sandwich ELISA (STJE0004104)
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产品名称:
Human NRG1 (Neuregulin 1) Sandwich ELISA (STJE0004104)
产品型号:
STJE0004104
产品展商:
ST john's laboratory
产品文档:
无相关文档
简单介绍
Human NRG1 (Neuregulin 1) Sandwich ELISA (STJE0004104)
产品描述
Applications:
ELISA
Reactivity:
Human
Note:
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:
9.38pg/mL
Detection Limit:
15.63~1000pg/mL
Short Description:
This NRG1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction:
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:
3.5h
Detection:
Colormetric
Gene Symbol:
NRG1
Gene ID:
3084
Uniprot ID:
NRG1_HUMAN
Specificity:
This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
Sample Type:
Serum, plasma and other biological fluids
Post Translational Modifications
Proteolytic cleavage close to the plasma membrane on the external face leads to the release of the soluble growth factor form. N- and O-glycosylated. Extensive glycosylation precedes the proteolytic cleavage.
Function
Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells.inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction.stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells.stimulating Schwann cell proliferation.implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development. Binds to ERBB4. Binds to ERBB3. Acts as a ligand for integrins and binds (via EGF domain) to integrins ITGAV:ITGB3 or ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and ERRB3 are essential for NRG1-ERBB signaling. Induces the phosphorylation and activation of MAPK3/ERK1, MAPK1/ERK2 and AKT1. Ligand-dependent ERBB4 endocytosis is essential for the NRG1-mediated activation of these kinases in neurons.
Protein Name
Pro-Neuregulin-1 - Membrane-Bound Isoform
Pro-Nrg1 Cleaved Into - Neuregulin-1
Acetylcholine Receptor-Inducing Activity
Aria
Breast Cancer Cell Differentiation Factor P45
Glial Growth Factor
Heregulin
Hrg
Neu Differentiation Factor
Sensory And Motor Neuron-Derived Factor
Database Links
Reactome: R-HSA-1227986 Q02297-10
Reactome: R-HSA-1236394 Q02297-10
Reactome: R-HSA-1250196 Q02297-10
Reactome: R-HSA-1250342
Reactome: R-HSA-1250347
Reactome: R-HSA-1251985
Reactome: R-HSA-1257604 Q02297-10
Reactome: R-HSA-1306955 Q02297-10
Reactome: R-HSA-1358803 Q02297-10
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642 Q02297-10
Reactome: R-HSA-2219530 Q02297-10
Reactome: R-HSA-5673001 Q02297-10
Reactome: R-HSA-6785631 Q02297-10
Reactome: R-HSA-6811558 Q02297-10
Reactome: R-HSA-8847993 Q02297-10
Reactome: R-HSA-8863795 Q02297-10
Reactome: R-HSA-9620244
Reactome: R-HSA-9664565 Q02297-10
Reactome: R-HSA-9665686 Q02297-10
Cellular Localisation
Pro-Neuregulin-1
Membrane-Bound Isoform: Cell Membrane
Single-Pass Type I Membrane Protein
Does Not Seem To Be Active
Neuregulin-1: Secreted
Isoform 8: Nucleus
May Be Nuclear
Isoform 9: Secreted
Has A Signal Peptide
Isoform 10: Membrane
May Possess An Internal Uncleaved Signal Sequence
Alternative ELISA Names
Pro-Neuregulin-1 - Membrane-Bound Isoform ELISA kit
Pro-Nrg1 Cleaved Into - Neuregulin-1 ELISA kit
Acetylcholine Receptor-Inducing Activity ELISA kit
Aria ELISA kit
Breast Cancer Cell Differentiation Factor P45 ELISA kit
Glial Growth Factor ELISA kit
Heregulin ELISA kit
Hrg ELISA kit
Neu Differentiation Factor ELISA kit
Sensory And Motor Neuron-Derived Factor ELISA kit
NRG1 ELISA kit
GGF ELISA kit
HGL ELISA kit
HRGA ELISA kit
NDF ELISA kit
SMDF ELISA kit
Specificity
This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
Reproducibility
Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable)
96T: 8 wells ×12 strips strips
-20℃, 6 months
Reference Standard
96T: 2 vials 48T: 1 vial
-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)
96T: 1 vial, 120 μL 60 μL
-20℃, 6 months
Concentrated HRP Conjugate (100×)
96T: 1 vial, 120 μL 60 μL
-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
2-8°C, 6 months
HRP Conjugate Diluent
1 vial, 14 mL
2-8°C, 6 months
Concentrated Wash Buffer (25×)
1 vial, 30 mL
2-8°C, 6 months
Substrate Reagent
1 vial, 10 mL
2-8℃ (Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
Sample Type Range (%) Average Recovery (%)
Serum(n=8)
85-97
90
EDTA plasma (n=8)
97-110
104
Cell culture media (n=8)
92-104
99
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample
1.00
2.00
3.00
1.00
2.00
3.00
n
20.00
20.00
20.00
20.00
20.00
20.00
Mean (pg/mL)
49.09
112.92
495.58
45.27
108.53
485.65
Standard deviation
2.76
5.33
23.79
2.72
5.21
21.32
CV (%)
5.62
4.72
4.80
6.01
4.80
4.39
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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