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Human NRG4 (Neuregulin 4) Sandwich ELISA (STJE0002563)

Human NRG4 (Neuregulin 4) Sandwich ELISA (STJE0002563)
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  • 产品名称:Human NRG4 (Neuregulin 4) Sandwich ELISA (STJE0002563)
  • 产品型号:STJE0002563
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human NRG4 (Neuregulin 4) Sandwich ELISA (STJE0002563)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.47ng/mL
Detection Limit: 0.78~50ng/mL
Short Description: This NRG4 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: NRG4
Gene ID: 145957
Uniprot ID: NRG4_HUMAN
Specificity: This kit recognizes Human NRG-4 in samples. No significant cross-reactivity or interference between Human NRG-4 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Low affinity ligand for the ERBB4 tyrosine kinase receptor. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. Does not bind to the ERBB1, ERBB2 and ERBB3 receptors.
Protein Name Pro-Neuregulin-4 - Membrane-Bound Isoform
Pro-Nrg4 Cleaved Into - Neuregulin-4
Nrg-4
Database Links Reactome: R-HSA-1227986
Reactome: R-HSA-1236394
Reactome: R-HSA-1250196
Reactome: R-HSA-1250342
Reactome: R-HSA-1250347
Reactome: R-HSA-1251985
Reactome: R-HSA-1257604
Reactome: R-HSA-1963640
Reactome: R-HSA-1963642
Reactome: R-HSA-2219530
Reactome: R-HSA-5673001
Reactome: R-HSA-6785631
Reactome: R-HSA-6811558
Reactome: R-HSA-8847993
Reactome: R-HSA-8863795
Reactome: R-HSA-9664565
Reactome: R-HSA-9665686
Cellular Localisation Pro-Neuregulin-4
Membrane-Bound Isoform: Cell Membrane
Single-Pass Type I Membrane Protein
Does Not Seem To Be Active
Neuregulin-4: Secreted
Alternative ELISA Names Pro-Neuregulin-4 - Membrane-Bound Isoform ELISA kit
Pro-Nrg4 Cleaved Into - Neuregulin-4 ELISA kit
Nrg-4 ELISA kit
NRG4 ELISA kit
Specificity This kit recognizes Human NRG-4 in samples. No significant cross-reactivity or interference between Human NRG-4 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-109 101
EDTA plasma (n=8) 91-104 97
Cell culture media (n=8) 93-105 99

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 2.50 5.60 17.49 2.35 6.02 18.70
Standard deviation 0.13 0.24 0.60 0.16 0.27 0.80
CV (%) 5.20 4.29 3.43 6.81 4.49 4.28
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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