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Human NRP1 (Neuropilin 1) Sandwich ELISA (STJE0019698)
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Human NRP1 (Neuropilin 1) Sandwich ELISA (STJE0019698)
产品资料
Human NRP1 (Neuropilin 1) Sandwich ELISA (STJE0019698)
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产品名称:
Human NRP1 (Neuropilin 1) Sandwich ELISA (STJE0019698)
产品型号:
STJE0019698
产品展商:
ST john's laboratory
产品文档:
无相关文档
简单介绍
Human NRP1 (Neuropilin 1) Sandwich ELISA (STJE0019698)
产品描述
Applications:
ELISA
Reactivity:
Human
Note:
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:
9.38pg/mL
Detection Limit:
15.63-1000pg/mL
Short Description:
This NRP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction:
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:
3.5h
Detection:
Colormetric
Gene Symbol:
NRP1
Gene ID:
8829
Uniprot ID:
NRP1_HUMAN
Specificity:
This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed.
Sample Type:
Serum, plasma and other biological fluids
Function
Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins. Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB. Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection. Isoform 2: Binds VEGF-165 and may inhibit its binding to cells. May induce apoptosis by sequestering VEGF-165. May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity.
Protein Name
Neuropilin-1
Vascular Endothelial Cell Growth Factor 165 Receptor
Cd Antigen Cd304
Database Links
Reactome: R-HSA-194306
Reactome: R-HSA-376176
Reactome: R-HSA-399954
Reactome: R-HSA-399955
Reactome: R-HSA-399956
Reactome: R-HSA-445144
Reactome: R-HSA-447041
Reactome: R-HSA-9694614
Cellular Localisation
Isoform 2: Secreted
Mitochondrion Membrane
Single-Pass Type I Membrane Protein
Cell Membrane
Cytoplasm
Alternative ELISA Names
Neuropilin-1 ELISA kit
Vascular Endothelial Cell Growth Factor 165 Receptor ELISA kit
Cd Antigen Cd304 ELISA kit
NRP1 ELISA kit
NRP ELISA kit
VEGF165R ELISA kit
Specificity
This kit recognizes Human NRP1 in samples. No significant cross-reactivity or interference between Human NRP1 and analogues was observed.
Reproducibility
Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable)
96T: 8 wells ×12 strips strips
-20℃, 6 months
Reference Standard
96T: 2 vials 48T: 1 vial
-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)
96T: 1 vial, 120 μL 60 μL
-20℃, 6 months
Concentrated HRP Conjugate (100×)
96T: 1 vial, 120 μL 60 μL
-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
2-8°C, 6 months
HRP Conjugate Diluent
1 vial, 14 mL
2-8°C, 6 months
Concentrated Wash Buffer (25×)
1 vial, 30 mL
2-8°C, 6 months
Substrate Reagent
1 vial, 10 mL
2-8℃ (Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
Sample Type Range (%) Average Recovery (%)
Serum(n=8)
90-107
98
EDTA plasma (n=8)
84-95
90
Cell culture media (n=8)
87-101
94
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample
1.00
2.00
3.00
1.00
2.00
3.00
n
20.00
20.00
20.00
20.00
20.00
20.00
Mean (pg/mL)
54.49
119.92
425.81
59.43
125.36
427.44
Standard deviation
3.81
5.35
21.76
4.08
5.73
22.40
CV (%)
7.00
4.46
5.11
6.86
4.57
5.24
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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