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Human PCAD (Cadherin, Placental) Sandwich ELISA (STJE0019874)

Human PCAD (Cadherin, Placental) Sandwich ELISA (STJE0019874)
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  • 产品名称:Human PCAD (Cadherin, Placental) Sandwich ELISA (STJE0019874)
  • 产品型号:STJE0019874
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human PCAD (Cadherin, Placental) Sandwich ELISA (STJE0019874)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 0.15 ng/mL
Detection Limit: 0.31-20 ng/mL
Short Description: This PCAD Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: CDH3
Gene ID: 1001
Uniprot ID: CADH3_HUMAN
Specificity: This kit recognizes Human PCAD in samples. No significant cross-reactivity or interference between Human PCAD and analogues was observed.
Sample Type: serum, plasma and other biological fluids
Tissue Specificity Expressed in some normal epithelial tissues and in some carcinoma cell lines.
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells.cadherins may thus contribute to the sorting of heterogeneous cell types.
Protein Name Cadherin-3
Placental Cadherin
P-Cadherin
Database Links Reactome: R-HSA-418990
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative ELISA Names Cadherin-3 ELISA kit
Placental Cadherin ELISA kit
P-Cadherin ELISA kit
CDH3 ELISA kit
CDHP ELISA kit
Specificity This kit recognizes Human PCAD in samples. No significant cross-reactivity or interference between Human PCAD and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-108 102
EDTA plasma (n=8) 93-110 100
Cell culture media (n=8) 86-98 91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 1.06 2.05 8.07 1.16 2.23 8.17
Standard deviation 0.06 0.11 0.40 0.07 0.10 0.41
CV (%) 5.74 5.33 4.95 5.90 4.67 5.06
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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