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Human PRB2 (Basic salivary proline-rich protein 2) CLIA Kit (STJC0000528)

Human PRB2 (Basic salivary proline-rich protein 2) CLIA Kit (STJC0000528)
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  • 产品名称:Human PRB2 (Basic salivary proline-rich protein 2) CLIA Kit (STJC0000528)
  • 产品型号:STJC0000528
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human PRB2 (Basic salivary proline-rich protein 2) CLIA Kit (STJC0000528)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human PRB2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: PRB2
Gene ID: 653247
Uniprot ID: PRB2_HUMAN
Specificity: This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications N- and O-glycosylated. In head and neck cancer patients, O-glycosylated with glucosylgalactosyl carbohydrate moiety. This modification would require prior hydroxylation on the lysine residue. Proteolytically cleaved at the tripeptide Xaa-Pro-Gln, where Xaa in the P(3) position is mostly lysine. The endoprotease may be of microbial origin. Pyroglutamate formation occurs on terminal Gln residues of cleaved peptides. Pyroglutamate formation found on at least Gln-398 and Gln-400.
Protein Name Basic Salivary Proline-Rich Protein 2
Salivary Proline-Rich Protein
Con1 Glycoprotein Cleaved Into - Basic Proline-Rich Peptide Ib-1 - Basic Proline-Rich Peptide P-E
Ib-9 - Basic Proline-Rich Peptide Ib-7 - Basic Proline-Rich Peptide Ib-8c
Basic Peptide P-F - Basic Proline-Rich Peptide Ib-4
Cellular Localisation Secreted
Alternative CLIA Names Basic Salivary Proline-Rich Protein 2 CLIA kit
Salivary Proline-Rich Protein CLIA kit
Con1 Glycoprotein Cleaved Into - Basic Proline-Rich Peptide Ib-1 - Basic Proline-Rich Peptide P-E CLIA kit
Ib-9 - Basic Proline-Rich Peptide Ib-7 - Basic Proline-Rich Peptide Ib-8c CLIA kit
Basic Peptide P-F - Basic Proline-Rich Peptide Ib-4 CLIA kit
PRB2 CLIA kit
Specificity This kit recognizes Human PRB2 in samples. No significant cross-reactivity or interference between Human PRB2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 100-116 107
EDTA plasma(n=8) 90-105 97
Cell culture media(n=8) 88-100 94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 54.95 148.39 477.86 55.21 147.30 457.85
Standard deviation 6.12 15.51 30.30 5.15 15.83 44.92
CV (%) 11.14 10.45 6.34 9.33 10.75 9.81
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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